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Clontech/Trusted chemistries for Apollo automation/96 Rxns/R400492

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TheApolloLibraryPrepSystemcombinesautomatedprecisionliquidhandlingwithgold-standardchemistries,suchasSMART-Seqv4andThruPLEX,todeliverthereliABIlity,flexibility,andreproducIBLeresultsyouexpect.YoucanalsoperformyourTruSeq®strandedmRNAlibraryprep,orgenerateVeriSeq™PGSlibraries,ontheApollosystemtomaximizeyourtimeandminimizesampleloss.TheApolloLibraryPrepSystemcombinesautomatedprecisionliquidhandlingwithgold-standardchemistries,suchasSMART-Seqv4andThruPLEX,todeliverthereliability,flexibility,andreproducibleresultsyouexpect.YoucanalsoperformyourTruSeq®strandedmRNAlibraryprep,orgenerateVeriSeq™PGSlibraries,ontheApollosystemtomaximizeyourtimeandminimizesampleloss.Preprogrammedscripts,includingthosevalidatedforrunningtheSMART-Seqv4reagentkit,aThruPLEXkit,IlluminaTruSeqStrandedmRNALibraryPrepkit,orVeriSeqPGSonthesystem,contributetosignificantlyreducedhands-ontimecomparedtomanuallibraryprepmethods.Simplysetup,hit[RUN],andwalkawaytoperformothertasks.SMART-Seqv4ReagentKitfortheApolloSystemThiskitisdesignedtogeneratehigh-quality,full-lengthCDNAdirectlyfrom1–1,000cellsor10pg–10ngoftotalRNAusingahigh-throughputworkflowthatenablesprocessingofupto96samplesinasingleworkingday.Withthiskit,wehavereformattedourbest-in-classmethodforultra-low-inputRNA-seqlibraryprepforautomationontheApolloplatform,providingawalkwaysolutionthatperformscomparablytomanualworkflows.cDNAproducedwiththiskitcanbeprocessedintoNGSlibrariessuitableforanalysisonIlluminaorIonTorrentplatforms.ThekitincorporatesthesametechnologiesandchemistriesassociatedwithourotherSMART-Seqv4products,includingoligo(dT)-priming,SMARTtemplate-switchingtechnology,andlockednucleicacids(LNA),ensuringtheproductionofcDNAlibrariesthatprovidefull-lengthsequenceinformation,accuraterepresentationofGC-richtranscripts,andindustry-leADIngsensitivity.ThruPLEXDNALibraryPrepontheApolloSystemWeadaptedourtrustedThruPLEXchemistryforautomationontheApollosystem,enablingyoutostartwith50pg–30ngoffragmenteddouble-strandedDNAor1–30ngofcfDNAandefficientlyprocessupto48samplesperrun,withoutwastingreagents.Thisversatilekit,combinedwiththeconvenienceandconsistencyoftheApollosystem,providesaddedsensitivitytobetterunderstandgeneticvariationswithinfragmentedDNAsamplesfrombiofluidssuchascell-freeDNA,genomicDNA,DNAfromFFPEmaterials,cDNA,orChIPDNA.Oncepurifiedandquantified,theresultinglibraryisreadyforIlluminaNGSinstrumentsusingstandardIlluminasequencingreagentsandprotocols.AutomatedTruSeqStrandedmRNALibraryPrepWorkflowontheApolloSystemNeverperformIlluminaTruSeqstrandedmRNAlibraryprepbyhandagain.Ourautomatedprotocol(downloadprotocol)adaptstheTruSeqStrandedmRNAlibraryprepprotocolfortheApollosystem,allowingyoutostartwith0.1–1µgoftotalRNAor10–100ngofpreviouslyisolatedmRNA(fromspecieswithpolyAtails)andprocessupto48samplesperrun,inmultiplesofsix(IlluminaCat.#20020594forlow-throughputorCat.#20020595forhigh-throughputworkflows).Oncepurifiedandquantified,theresultinglibraryisreadyforsequencingonIlluminaNGSinstrumentsusingstandardIlluminasequencingreagentsandprotocols.AutomatedVeriSeqLibraryPrepforPGSontheApolloSystemScreenall24chromosomesforaneuploidywiththeIlluminaVeriSeqPGSKit(IlluminaCat.#RH-101-1001)usingourautomatedprotocol(downloadprotocol).ThisworkflowleveragestheApollosystem"sprecisionautomationtoallowthegenerationofupto96VeriSeqlibrariesin13hours,increasingreliabilityandefficiencybydecreasingvariabilityandhumanerror.Followinglibrarygenerationandnormalization,theresultinglibrariesarereadyforIlluminaNGSusingstandardIlluminasequencingreagentsandprotocols. More Less

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我们是Takara Bio Inc.的一部分，Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂（这是同类工厂中的第一个）能够对病毒，细胞和蛋白质进行无缝和无菌处理。我们的北美（NA）总部位于加利福尼亚山景城，我们在这里从事大部分研发，营销，质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究，物流和外展计划做出了贡献。此外，我们在瑞典哥德堡拥有GMP干细胞设施，在法国的Takara Bio Europe总部也为欧洲，中东和非洲的部分地区提供服务。