产品说明
Strand-specific RNA-seq libraries for Illumina platforms from high-input total RNAThe SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines RiboGone and SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation in around 5 hours.Strand-specific RNA-seq libraries for Illumina platforms from high-input total RNAThe SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combinesRiboGoneand SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation in around 5 hours. Because rRNA can comprise ~90% or more of total RNA, effective depletion of rRNA prior to library generation can lower sequencing costs and improve mapping statistics. SMART cDNA synthesis technology followed by PCR complete a streamlined process that performs strand information and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and amplification steps. These high-input kits contain 12 reverse primers and up to 8 forward primers that allow for multiplexing of up to 96 separate libraries in a high-throughput format. The specific primers you receive will depend on your particular kit. The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only about 5 hours.Alternate kits for other input amounts:For 250 pg–10 ng of total RNA, we recommend the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input MammalianFor 10–100 ng of total RNA, we recommend the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian More Less
50多年来,Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂,仪器和服务产品组合支持生命科学领域的进步,包括一线诊断和临床应用。
我们是Takara Bio Inc.的一部分,Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂(这是同类工厂中的第一个)能够对病毒,细胞和蛋白质进行无缝和无菌处理。我们的北美(NA)总部位于加利福尼亚山景城,我们在这里从事大部分研发,营销,质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究,物流和外展计划做出了贡献。此外,我们在瑞典哥德堡拥有GMP干细胞设施,在法国的Takara Bio Europe总部也为欧洲,中东和非洲的部分地区提供服务。
新品排行榜
1
Clontech/Plasmid maxipreps—Nucle...
2
Clontech/Single-cell library pre...
3
Clontech/ProteoTuner expression ...
4
Clontech/Random Primer DNA Label...
5
Clontech/ThruPLEX DNA-Seq Kit: a...
6
Clontech/Rapid Capturem Protein ...
7
Clontech/GMP recombinant Cas9/0....
8
Clontech/Legacy RNA-seq kits/96 ...
9
Clontech/Legacy DNA-seq kits/48 ...
10
Clontech/CloneAmp HiFi PCR Premi...
微信公众号

手机扫码,关注动态