产品概述 Abcam s Gold Conjugation Kit allows antibodies or proteins to be covalently attached to ultra-stable Gold nanoparticles at very high OD quickly and easily. The nanoparticles in the Abcam s Gold Conjugation Kit have a protective surface coat that can withstand the most extreme conditions (e.g. 2.5M NaOH at 70 C for 1 hour). The hands-on time for the Abcam Gold conjugation procedure is around 2 minutes and the conjugate is ready to use within 20 minutes. The researcher simply pipettes the biomolecule into a vial containing the nanoparticles of the Gold Conjugation Kit.The nanoparticles in this kit are supplied as a freeze-dried mixture. The conjugation reaction is initiated simply by reconstituting the freeze-dried nanoparticles with the antibody, which becomes attached (through lysine residues) to the surface of the nanoparticles.The resulting covalent conjugates are more stable than those prepared by passive adsorption methods. Moreover, unlike passive methods, the coating process is independent of the isoelectric point of the antibody, avoiding the need for extensive trials at different pH values. All antibodies can be labelled at a single pH.Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.BenefitsEasy and rapid conjugation only 2 minutes hands-on time and 100% recovery of materialsSite-specific labelling Antigen binding sites of antibodies are free to bind the target moleculeProprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the Gold Stable conjugates formedFully scalable Easy transfer from R D to manufacturingUniform spherical shape and narrow size distribution Consistent high quality and excellent batch-to-batch reproducibilityBuffer requirements:The biomolecule to be conjugated should ideally be in 10 mM amine-free buffer (e.g. MES, MOPS, HEPES), pH range 6.5 to 8.5. Sugars have no effect on conjugation efficiency. For incompatible buffers and low antibody concentrations, use our rapidantibody purification and concentration kitsfor Nanoparticles. To learn more about incompatible buffers, please refer to the protocol booklet. This product is manufactured by Expedeon, an Abcam company, and was previously called 20nm InnovaCoat GOLD. 229-0015 is the same as the 1 x 25 g size. 229-0010 is the same as the 10 x 2.5 g size. 229-0005 is the same as the 3 x 2.5 g size.The 3 and 10 Test Conjugation Kits are designed to label 12 l of antibody per vial. The 1 Test Conjugation Kit is designed to label 120 l of antibody per vial. Lateral flow assay - Gold Conjugation Kit (20 nm, 20 OD) (ab188215)Image from Gwyn et al., J Immunol Methods, 435:27-31; doi: 10.1016/j.jim.2016.05.008. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/ Gwyn, Sarah, et al used Gold Conjugation Kit (20 nm, 20 OD) (ab188215) as part of examining the detection of antibodies against immunodominant antigen Pgp3 from Chlamydia trachomatis. They used the kit to conjugate Gold to Pgp3 protein for use in lateral flow assay.LFA test. Shown are results of the LFA run with positive (left) and negative (right) control sera. 发表研究结果有使用 ab188215？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab188215 被引用在 9 文献中. Foggetti A et al. Spiny and Non-spiny Parvalbumin-Positive Hippocampal Interneurons Show Different Plastic Properties. Cell Rep 27:3725-3732.e5 (2019).PubMed: 31242406 Depannemaecker D et al. Gold Nanoparticles for X-ray Microtomography of Neurons. ACS Chem Neurosci 10:3404-3408 (2019).PubMed: 31274276 Jiang Q et al. Paper-Based Microfluidic Device (DON-Chip) for Rapid and Low-Cost Deoxynivalenol Quantification in Food, Feed, and Feed Ingredients. ACS Sens 4:3072-3079 (2019).PubMed: 31713421 Cabezón I et al. Serial block-face scanning electron microscopy applied to study the trafficking of 8D3-coated gold nanoparticles at the blood-brain barrier. Histochem Cell Biol 148:3-12 (2017).PubMed: 28283744 Anderson CE et al. Rapid Diagnostic Assay for Intact Influenza Virus Using a High Affinity Hemagglutinin Binding Protein. Anal Chem 89:6608-6615 (2017).PubMed: 28499086 Ortega Arroyo J et al. Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging. Nat Protoc 11:617-33 (2016).PubMed: 26938114 Gwyn S et al. Lateral flow-based antibody testing for Chlamydia trachomatis. J Immunol Methods 435:27-31 (2016).PubMed: 27208400 Cabezón I et al. Trafficking of Gold Nanoparticles Coated with the 8D3 Anti-Transferrin Receptor Antibody at the Mouse Blood-Brain Barrier. Mol Pharm 12:4137-45 (2015).PubMed: 26440359 Wang HK et al. Cellulose-Based Diagnostic Devices for Diagnosing Serotype-2 Dengue Fever in Human Serum. Adv Healthc Mater : (2013).PubMed: 23843297 There are currently no Customer reviews or Questions for ab188215.Please use the links above to contact us or submit feedback about this product.Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com