Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [EPR6518] to JunB - BSA and Azide free Suitable for: ICC/IF, IP, WB Reacts with: Mouse, Rat, Human Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. WB: MCF7, Human tonsil, Human uterus cancer, mouse uterus, rat lung, rat spleen, HeLa and HACAT lysates ICC/IF: HeLa, and MCF7 cells. IP: HeLa cells. 常规说明 ab232033 is the carrier-free version of ab128878.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar is a trademark of Fluidigm Canada Inc.This product is a recombinant monoclonal antibody, which offers several advantages including:- High batch-to-batch consistency and reproducibility- Improved sensitivity and specificity- Long-term security of supply- Animal-free productionFor more information see here.Our RabMAb technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb patents.We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated \'PUR\' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team. The Abpromise guarantee Abpromise™承诺保证使用ab232033于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 ab128878 (purified) at 1:100 dilution (2 g) immunoprecipitating JunB in HeLa whole cell lysates.Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug Lane 2 (+): ab128878 HeLa whole cell lysatesLane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878). Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1:200 dilution (9.1 g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor 594) 1:200 (2.5 g/ml). Goat anti rabbit IgG (Alexa Fluor 488, ab150077) was used as the secondary antibody at 1:1000 (2 g/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878). Unpurified ab128878, at a 1/250 dilution, staining JunB in MCF7 (Human breast adenocarcinoma cell line) cells by Immunofluorescence.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878). Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128878). To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on. Click here to view the general protocols To download a Certificate of Compliance, please enter your Lot number below:Lot number 发表研究结果有使用 ab232033？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab232033 尚未被引用在任何文献中。 There are currently no Customer reviews or Questions for ab232033.Please use the links above to contact us or submit feedback about this product.Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com