Full length native protein (purified) corresponding to Human Myc tag conjugated to keyhole limpet haemocyanin.Database link: P01106(Peptide available as ab13837) Run BLAST withRun BLAST with 常规说明 Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As 存储溶液 pH: 6.8Preservative: 0.1% Sodium azideConstituents: 0.0268% PBS, 0.58% Sodium chloride The Abpromise guarantee Abpromise™承诺保证使用ab1253于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. IPUse a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. 发表研究结果有使用 ab1253？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab1253 被引用在 11 文献中. Strutt H Strutt D DAnkrd49 and Bdbt act via Casein kinase Ie to regulate planar polarity in Drosophila. PLoS Genet 16:e1008820 (2020).PubMed: 32750048 Huang Z et al. PTPN2 regulates the activation of KRAS and plays a critical role in proliferation and survival of KRAS-driven cancer cells. J Biol Chem 295:18343-18354 (2020).PubMed: 33122197 Katoh I et al. C-terminal a Domain of p63 Binds to p300 to Coactivate ß-Catenin. Neoplasia 21:494-503 (2019).PubMed: 30986748 Cai L et al. Identification of a genetic interaction between the tumor suppressor EAF2 and the retinoblastoma protein (Rb) signaling pathway in C. elegans and prostate cancer cells. Biochem Biophys Res Commun 447:292-8 (2014).PubMed: 24727455 Carballo JA et al. Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery. PLoS Genet 9:e1003545 (2013).PubMed: 23825959 View all Publications for this product Sample Trypanosoma brucei Cell lysate - other (Abcam beads (ab1253) Anti-myc developed in goat) 1) Blocking step Milk as blocking agent for 30 minute(s) Concentration: 5% Temperature: 23 CYou don\'t need to wash the beads prior to using, and perform the IP at 4C. Also, use 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. Below is a general IP protocol that the originator of this antibody sent that you can use as a guideline. If you have any additional questions, please contact us again.Immunoprecipitation Protocol For use with Antibodies Immobilized on Agarose:A. To microcentrifuge tube add:1) 15 to 25 ul of slurry of agarose immobilized Ab (3.75 to 6.125 ug Ab)2)500 ul 2x Stringent IP buffer*:100 mM Tris-HCl (pH 8.0)1000 mM NaclChelating agents:2 mM EDTA and/or 2 mM EDTADetergents:2 % IGEPAL CA-630 (replacement for NP40) 0.2 % SDS2 % DeoxycholateProtease Inhibitors:20 ug/ml Aprotinin20 ug/ml Leupeptin2 mM PMSF or 10 ug/ml Pepstatin A or 8 mM AEBSFPhosphatase Inhibitors – If Necessary:200 mM NaF 2 mM Na3VO450 mM ?-Glycerophosphate 3)Cell lysate (0.1 to 1.0 mg total protein)4)diH2O to a total volume of 1 mlB. Vortex and incubate for between 1 and 24 h at 4 deg. Turning tubes on a rotating platform wheel allows continuous mixing.C. Centrifuge (16,000 x g, 3 to 5 min), aspirate and discard supernatantD. Wash pellet in 1x IP buffer 3 to 6 times. Washing consists of resuspending pellet, centrifuging (16,000 x g, 3 to 5 min), aspirating and discarding supernatant.E. After last wash, resuspend pellet in 30 ul 2x Electrophoresis Sample Buffer, boil 5 min. F. Centrifuge, load supernatant onto SDS-PAGE gel, electroblot onto PVDF membrane and perform Western Blot Analysis.*Buffers for Immunoprecipitation AssaysFor analysis of a single protein without associated proteins, the use of high ionic strength, strong detergents, and rigorous washing of the immunoprecipitate are recommended. One suitable buffer is the Strigent IP buffer outlined above.For co-precipitation of associated proteins, the use of near physiological ionic strength and gentle detergents is recommended. One suitable buffer is the Lenient IP buffer outlined below.The buffers outlined represent two extremes. The optimal buffer for ones given application must be empirically defined. This buffer may be of intermediate ionic strength and/or detergent composition.1x Lenient IP buffer:50 mM Tris-HCl (pH 8.0)100 mM NaclChelating agents:1 mM EDTA and/or 1 mM EGTADetergents:0.5 % IGEPAL CA-630 (replacement for Nonidet P40)Protease Inhibitors:10 ug/ml Aprotinin10 ug/ml Leupeptin1 mM PMSF or 5 ug/ml Pepstatin A or 4 mM AEBSFPhosphatase Inhibitors – If Necessary:100 mM NaF1 mM Na3VO450 mM ?-Glycerophosphate Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com