Mouse, Human, Saccharomyces cerevisiae
预测可用于: Vertebrata
ab24609 recognizes the conserved domain FXFG repeats in nucleaporins like the p62, p152, p90.

This is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.

This product was changed from ascites to tissue culture supernatant on 20/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As

存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Primary antibody说明 This is a reliable general purpose monoclonal antibody which recognizesa related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.
The Abpromise guarantee

Abpromise™承诺保证使用ab24609于以下的经测试应用

\"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

Use at an assay dependent concentration.

Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer.

ICC/IF
Use at an assay dependent concentration.

Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer.

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
细胞定位 Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
Immunocytochemistry/ Immunofluorescence - Anti-Nup107 antibody [Mab414] (ab24609)Image courtesy of Saram N J et al. J Biol Chem. 2011 Nov 11; 286(45): 38989–39001. doi: 10.1074/jbc.M111.297952

ab24609 staining Nup107 inHuman chronic myelogenous leukemia cells from bone marrow cells. Cells were fixed with 4% paraformaldehyde inDPBS buffer for 20 minsand permeabilised with 0.1% Triton X-100 for 20 min at room temperature. Cells wereblocked with 2% normal goat serum in DPBS with 1% BSA and washed with 0.1% Tween 20.

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-Nup107 antibody [Mab414] (ab24609)This image is courtesy of an Abreview submitted by Dr Kirk McManus

ab24609 (1/200) staininig Nup107 in human RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100 and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

This image was generated using the ascites version of the product.

See Abreview

Immunocytochemistry/ Immunofluorescence - Anti-Nup107 antibody [Mab414] (ab24609)Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome

ab24609 (1/500) staining Nup107 in human Hela Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T C) or Methanol (6 min in Methanol -20 C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-Nup107 antibody [Mab414] (ab24609)Image and protocol kindly provided by Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome

ab24609 (1/500) staining Nup107 in murine NIH/3T3 Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T C) or Methanol (6 min in Methanol -20 C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

This image was generated using the ascites version of the product.

发表研究结果有使用 ab24609？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab24609 被引用在 113 文献中.

Di Stefano M et al. Impact of Chromosome Fusions on 3D Genome Organization and Gene Expression in Budding Yeast. Genetics 214:651-667 (2020).PubMed: 31907200 Huber S et al. Recruitment of Host Nuclear Pore Components to the Vicinity of Theileria Schizonts. mSphere 5:N/A (2020).PubMed: 32024710 Agote-Aran A et al. Spatial control of nucleoporin condensation by fragile X-related proteins. EMBO J 39:e104467 (2020).PubMed: 32706158 Lee ES et al. TPR is required for the efficient nuclear export of mRNAs and lncRNAs from short and intron-poor genes. Nucleic Acids Res 48:11645-11663 (2020).PubMed: 33091126 Zila V et al. Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells. mBio 10:N/A (2019).PubMed: 31690677 View all Publications for this product
Blocking step BSA as blocking agent for 30 minute(s) Concentration: 1% Temperature: 25 C
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 1% Temperature: 21 C
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) Concentration: 5% Temperature: 21 C
Blocking step FCS in PBS as blocking agent for 30 minute(s) Concentration: 10% Temperature: 22 C

The staining pattern of this antibody should be nuclear or nuclear membrane.

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While this product is predicted to react with all vertebrates, we have not yet tested this with cow. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:



The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.





I hope this information is helpful. Please let me know if you would like to participate in this program and test in cow. Also, please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

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I am very pleased to hear you would like to accept our offer and test one or both of these products in blocking experiements. Each code is associated with a paricular antibody so be sure tho reference the correct code when ordering and submitting the Abreview. Each will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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