Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [EP611Y] to NAK/TBK1 Suitable for: ICC/IF, WB, IHC-P Knockout validated Reacts with: Mouse, Rat, Human Synthetic peptide within Human NAK/TBK1 aa 1-100 (N terminal). The exact sequence is proprietary. WB: HeLa membrane extract lysate (ab29547), HepG2, SH-SY5Y, C6, HAP1 and NIH/3T3 cell lysateIHC-P: Human hepatocellular carcinomaICC/IF: MCF7 cells 常规说明 Anti-NAK/TBK1 antibody [EP611Y] (ab40676) may not be suitable for IHC with mouse or rat samples.This product is a recombinant monoclonal antibody, which offers several advantages including:- High batch-to-batch consistency and reproducibility- Improved sensitivity and specificity- Long-term security of supply- Animal-free productionFor more information see here.Our RabMAb technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb patents.We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated \'PUR\' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team. 存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. 存储溶液 pH: 7.20Preservative: 0.01% Sodium azideConstituents: 40% Glycerol, 0.05% BSA, 59% PBS The Abpromise guarantee Abpromise™承诺保证使用ab40676于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 ICC/IF 1/100. For unpurified use at 1/250 - 1/500. WB (1) 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). For unpurified use at 1/1000. WB1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). For unpurified use at 1/1000. IHC-P1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.For unpurified use at 1/50. Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively. 序列相似性 Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.Contains 1 protein kinase domain. Serine/threonine protein kinase TBK 1 antibody Serine/threonine protein kinase TBK1 antibody Serine/threonine-protein kinase TBK1 antibody T2K antibody TANK binding kinase 1 antibody TANK-binding kinase 1 antibody TBK 1 antibody Tbk1 antibody TBK1_HUMAN antibody see all Lane 1: Wild-type HAP1 cell lysate (20 g)Lane 2:NAKknockout HAP1 cell lysate (20 g) Lane 3:HeLacell lysate (20 g)Lane 4:HepG2cell lysate (20 g)Lanes 1 - 4: Merged signal (red and green). Green - ab40676 observed at 90kDa. Red - loading control, ab8245, observed at 37kDa.ab40676 was shown to specifically react with NAKwhen NAKknockout samples were used. Wild-type and NAKknockout samples were subjected to SDS-PAGE. ab40676 and ab8245(loading control to GAPDH) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4 C. Blots were developed with goat anti-rabbit IgG H L (IRDye 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H L (IRDye 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue sections labeling NAK/TBK1 with purified ab40676 at a dilution of 1/100 (11.5 g/ml). ab97051 Goat Anti-Rabbit IgG H L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. All lanes : Anti-NAK/TBK1 antibody [EP611Y] (ab40676) at 1/5000 dilution (purified)Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysateLane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysateLane 3 : C6 (Rat glial tumor cell line) whole cell lysateLane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilutionPredicted band size: 84 kDaObserved band size: 84 kDaBlocking/Diluting buffer 5% NFDM/TBST Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma) cells labeling NAK/TBK1 with purified ab40676 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluo 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor 594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control. To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on. Click here to view the general protocols 发表研究结果有使用 ab40676？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab40676 被引用在 79 文献中. Mukai K et al. Homeostatic regulation of STING by retrograde membrane traffic to the ER. Nat Commun 12:61 (2021).PubMed: 33397928 Banerjee A et al. Experimental and natural evidence of SARS-CoV-2-infection-induced activation of type I interferon responses. iScience 24:102477 (2021).PubMed: 33937724 Inoue Y et al. The stimulator of interferon genes (STING) pathway is upregulated in striatal astrocytes of patients with multiple system atrophy. Neurosci Lett 757:135972 (2021).PubMed: 34033888 Li C et al. RNF111-facilitated neddylation potentiates cGAS-mediated antiviral innate immune response. PLoS Pathog 17:e1009401 (2021).PubMed: 33720974 Chu L et al. Perillaldehyde Inhibition of cGAS Reduces dsDNA-Induced Interferon Response. Front Immunol 12:655637 (2021).PubMed: 33968056 View all Publications for this product Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 26 CPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com