Recombinant full length protein within Human ALP. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As

存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The Abpromise guarantee

Abpromise™承诺保证使用ab67228于以下的经测试应用

\"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

Actinin-associated LIM protein antibody Alpha actinin 2 associated LIM protein antibody
All lanes : Anti-ALP antibody (ab67228) at 1/500 dilution

Lane 1 : ALP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 39 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

发表研究结果有使用 ab67228？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab67228 被引用在 11 文献中.

Qiao W et al. miR-224-5p protects dental pulp stem cells from apoptosis by targeting Rac1. Exp Ther Med 19:9-18 (2020).PubMed: 31897093 Wang P et al. The COL6A1 rs201153092 single nucleotide polymorphism, associates with thoracic ossification of the posterior longitudinal ligament. Mol Med Rep 21:191-200 (2020).PubMed: 31939624 Chia W et al. A circular RNA derived from DAB1 promotes cell proliferation and osteogenic differentiation of BMSCs via RBPJ/DAB1 axis. Cell Death Dis 11:372 (2020).PubMed: 32415085 Gao R et al. Cyclic stretch promotes the ossification of ligamentum flavum by modulating the Indian hedgehog signaling pathway. Mol Med Rep 22:1119-1128 (2020).PubMed: 32626952 Li S et al. Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells. Cell Biosci 9:1 (2019).PubMed: 30622695 View all Publications for this product

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