By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected. 最小交叉反应 Chicken, Cow, Horse, Human, Mouse, Pig, Rat To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details 纯化说明 Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). The Abpromise guarantee Abpromise™承诺保证使用ab97080于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 ICC Use at an assay dependent concentration. IHC-P 1/200 - 1/5000. ELISA 1/25000 - 1/75000. as primary. WB (1) 1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000. WB1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000. IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3 g/ml overnight at +4 C. An HRP-conjugated secondary (Ab97080,1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.The inset negative control image is secondary-only at 1/500 dilution.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre Goat polyclonal Secondary Antibody to Rabbit IgG - H L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution developed using the ECL technique, with ab87342. To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on. Click here to view the general protocols 发表研究结果有使用 ab97080？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab97080 被引用在 100 文献中. Bazzar W et al. Pharmacological inactivation of CDK2 inhibits MYC/BCL-XL-driven leukemia in vivo through induction of cellular senescence. Cell Cycle 20:23-38 (2021).PubMed: 33356836 Susantad T et al. Modified recombinant human erythropoietin with potentially reduced immunogenicity. Sci Rep 11:1491 (2021).PubMed: 33452310 Li H et al. Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling. Aging (Albany NY) 13:4522-4551 (2021).PubMed: 33495420 Iacono D et al. Reduction of pTau and APP levels in mammalian brain after low-dose radiation. Sci Rep 11:2215 (2021).PubMed: 33500491 Pralle RS et al. Greater liver PNPLA3 protein abundance in vivo and in vitro supports lower triglyceride accumulation in dairy cows. Sci Rep 11:2839 (2021).PubMed: 33531537 View all Publications for this product This item was applied as a secondary Ab for my WB. 1:10,000 dilution was applied for chemiluminescence reaction afterwards. I also conducted the procedure with secondary Ab probing only (right side of the blot) and the result showed no non-specific binding for this Ab. I am satisfied for this item in my WB assay. Sections were thawed and fixed in cold acetone. Endogenous peroxidase and serum blocking (10% normal horse serum) were performed. Primary antibody was incubated with ab9722, at 5µg/ml overnight at 4°C. An HRP-conjugated secondary (ab97080 at 1/300 dilution) was used for 30min at room temperature. Sections were counterstained with haematoxylin and mounted with DPX.A negative control was included using an isotype IgG at the same concentration as the primary antibody.*Staining performed by Maria Wallert. Pr -adsorption est une tape suppl mentaire pour augmenter la sp cificit d\'un anticorps. Je vous envoie un lien pour notre site internet qui explique ce terme en plus de d tailes.https://www.Abcam.com/index.html?pageconfig=resource rid=12956 Read More Thank you for contacting us.Please have a look at the troubleshooting guide given on our website;https://www.abcam.com/index.html?pageconfig=resource rid=11384I am sorry I am unable to get the specific protocol for chicken tissues. My colleague from laboratory has suggested that any general IHC-P protocol would work and the staining can be improved with optimization.I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read MoreWhile I did not receive the image which you have referenced I would like to offer so help regarding your questions. Generally specific details regarding secondary antibody incubation and concentration as will need to be determined empirically in the lab. These may vary considerable depending upon the secondary antibody used. This is the same for exposure times. We do offer a number of guidelines for information on our datasheet however. Please remember that these are suggested starting points and you will likely need to adjust these to find the optimal ranges.The secondary antibody AB97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H L (HRP), pre-adsorbed (ab97080) was used at 1/5000 dilution. In this experiment ECL detection was used with a 20 minute exposure time. This product may be found at this location:https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-pre-adsorbed-ab97080.htmlThe secondary antibody AB65484 Goat polyclonal Secondary Antibody to Rabbit IgG - H L (HRP), pre-adsorbed (ab97080) was used at 1/5000 dilution. In this experiment ECL detection was used with a 150 second exposure time. Unfortunately this product is no longer available.A third experiment is shown on the datasheet; however we do not have specific information as to the product number of the secondary antibody used.I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote RABMAB-XBSMG in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource rid=15447 Read More Thank you for contacting us.Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing our antibodies at various temperatures of up to 45 C for 1 week does not impact their activity. I would be happy to share a summary of our findings with you; please let me know if you would like to see this.As a precautionary measure only, we ship our antibodies in packaging with ice packs to provide extra temperature stability in transit. Once you have received any products, we recommend toplease follow the long-term storage instructions on the datasheet as soon as possible. This will help to ensure that the product remains stable and that we can continue to provide our guarantee.In this case, considering our findings described above, I would suggest that the antibody should not be significantly affected from being at room temperature for 3 days. We recently redesigned our packing. A decision was taken to label these with \'Temperature sensitive - store according to datasheet instructions\' in order to cover all the different products we ship at different temperatures.This instruction should prompt the recipient to check the datasheet, or check with the end user, as soon as possible.However, I do understand your concerns, and we encourage feedback from customers and take this seriously. I have therefore forwarded your comments on to my colleagues for their reference.I would like to reassure you that our Abpromise to you is that, should our product not work as stated on the datasheet, we will resolve the issue to your satisfaction, either with helpful advice to optimize your experiment or a replacement or refund of the product. Please contact our knowledgeable Scientific Support staff should you experience any issues while using our products. Contact information can be found at:https://www.abcam.com/index.html?pageconfig=contactusI hope this information helps, but please don\'t hesitate to contact me for additional information or assistance. Read More Thank you for your email. I\'m glad to see that the antibody worked. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews Read More Thank you for your email. I\'d suggest starting with the recommended dilutions for each antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews Read More Thank you for your email. The free of charge order was shipped today for delivery tomorrow. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews Read More You are very welcome. I keep my fingers crossed for you and wish you good luck. Yes, definitely, please keep me informed. I look forward to hear back from you - hopefully with positive results. Have a nice weekend. Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com