Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [4.8] to C1q Suitable for: IHC-FoFr Knockout validated Reacts with: Mouse 常规说明 This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University:Alexander H. Stephan et al. , A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.Our RabMAb technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb patents.We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated \'PUR\' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team. 存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. The Abpromise guarantee Abpromise™承诺保证使用ab182451于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 IHC-FoFr (1) Use a concentration of 1 µg/ml. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. IHC-FoFrUse a concentration of 1 µg/ml. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. 疾病相关 Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. 翻译后修饰 O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Complement C1q subcomponent subunit A antibody Complement C1q subcomponent subunit B antibody Complement C1q subcomponent subunit C antibody Complement component 1 q subcomponent alpha polypeptide antibody Complement component C1q A chain antibody Complement component C1q B chain antibody Complement component C1q C chain antibody see all Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-C1q antibody [4.8] (ab182451)This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital. ab182451 (1/500) staining C1q in 15 m coronal brain sections from postnatal day 90 C1qA KO mice and WT litter-mates (Botto et al., 1998) by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton. An Alexa Flour-conjugated Goat anti-Mouse IgG polyclonal (1/500) was used as the secondary antibody. Positive staining on wild type (WT) mouse cerebral cortex, and no staining on knockout (KO) mouse cerebral cortex. To download a Certificate of Compliance, please enter your Lot number below:Lot number 发表研究结果有使用 ab182451？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab182451 被引用在 33 文献中. Fairley LH et al. Neuroprotective effect of mitochondrial translocator protein ligand in a mouse model of tauopathy. J Neuroinflammation 18:76 (2021).PubMed: 33740987 Salvany S et al. Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury. Glia 69:1216-1240 (2021).PubMed: 33386754 Frew J Nygaard HB Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice. Acta Neuropathol Commun 9:57 (2021).PubMed: 33795008 Clayton K et al. Plaque associated microglia hyper-secrete extracellular vesicles and accelerate tau propagation in a humanized APP mouse model. Mol Neurodegener 16:18 (2021).PubMed: 33752701 Zhang J et al. Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency. Nature 588:459-465 (2020).PubMed: 32866962 View all Publications for this product Blocking step BSA as blocking agent for 30 minute(s) Concentration: 1% Temperature: 24 C Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 2% Temperature: 24 C Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 24 C Blocking step BSA as blocking agent for 20 minute(s) Concentration: 10 g/mL Temperature: RT CPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com