本人在做有关生物素标记抗体，首先需要将生物素活化。我们用的生物素是sigma公司的B4501的生物素，需制成NHS-Biotin，请各位高手提供一下详细的实验步骤，以及NHS-Biotin与抗体标记的步骤，不胜感谢。请写明所查资料的出处，谢谢！跟楼主同感。手头有一篇，还没试。Electrogenerated Chemiluminescence. 80. C-Reactive Protein Determination at High Amplification with [Ru(bpy)3]2+-Containing Microspheres，supporting information里的:Biotinylation of Anti-CRP. Briefly, ~4.5 mg of sulfo-NHS-LC-biotin was added to 1 mL of ~ 90 mg/mL anti-CRP PBS solution (pH 7.2), and the reaction was allowed to proceedfor~30minatroomtemperature.Freebiotinmoleculesweresubsequently removed by a Sephadex G-25 PD-10 desalting column(Amersham Pharmacia Biotech, Piscataway,NJ).Thefinalproductwithaconcentrationof~15 mgbiotinylatedanti-CRP/mL in PBS (pH 7.2) was stored at ~4 ?C prior to use.引用回帖:1楼: Originally posted by 萌1986 at 2011-10-09 15:20:38:大家好~~~本人在做有关生物素标记抗体，首先需要将生物素活化。我们用的生物素是sigma公司的B4501的生物素，需制成NHS-Biotin，请各位高手提供一下详细的实验步骤，以及NHS-Biotin与抗体标记的步骤，不胜感谢 ... 网上版本太多，都不太一样，不知道哪一种比较合适~