Features & Benefits

Product Specifications

Performance Characteristics

Method: CompetitiveAnalyte: Intact osteocalcinSpecimen: SerumSpecimen Volume: 25 µLLOD*: 0.45 ng/LLLOQ*: 2 ng/mLHLOQ*: 32 ng/LPrecision CVs within-run: 5%-10%Precision CVs between-run: 5%-10%Incubation Time: 3.6 HoursCross-reactivity: Intact OC 100% Reduced OC 0% N-, C- peptides 0%

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96-well microplate with reagents sufficient to test 40 samples in duplicate

Species Reactivity

Rabbit, Pig, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture, Guinea PigNo recognition of Rat, Mouse, or Dog

Assay Steps

Add 25 µL Standards, Controls, and SamplesAdd 125 µL anti-Osteocalcin AntibodyIncubate 2 hours at room temperatureWash 3 times with 1X Wash BufferAdd 150 µL reconstituted Enzyme ConjugateIncubate 60 minutes at room temperatureWash 3 times with 1X Wash BufferAdd 150 µL Working Substrate SolutionIncubate 35-40 minutes at room temperatureAdd 50 µL Stop SolutionRead Optical Density at 405 nmData Reduction: 4 Parameter

Ordering Information

Product Documentation

Package Insert - English

Package Insert - English - CE

SDS

Technical Data Sheet

Technical Bulletin - ELISA Wash Technique

Package Insert - Danish

Package Insert - French

Package Insert - German

Package Insert - Italian

Package Insert - Portuguese

Package Insert - Spanish

Package Insert - Swedish