首页 > Viagen > Viagen/Proteinase K Solution 5ml (20mg/ml)/501-PK

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

Viagen/Proteinase K Solution 5ml (20mg/ml)/501-PK

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

501-PK

品牌：

Viagen

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

DetailsProteinase K is an endolytic protease that cleaves peptide bonds atthe carboxylic sides of aliphatic, aromatic or hydrophobic amino acids.Proteinase K is classified as a serine protease. The smallest peptide tobe hydrolysed is a tetrapeptide.DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biologicalsamples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR ReagentProtocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for~24 hrs.Other applications: Proteinase K digests nativeproteins very effectively. It can therefore be used to remove DNases andRNases when isolating DNA and RNA from tissues or cells.Usage Conditions: • Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5• Under denaturing conditions—e.g., in the presence of SDS or urea• In the presence of metal chelating agents—e.g., EDTA• At high temperatures—optimum digestion temperature is 65°CUnit Definition: One unit of the enzymeliberates folin-positive amino acids and peptides corresponding to 1μmoltyrosine in 1min at 37°C using denatured hemoglobin as substrate.Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases