ThismouseIgMkmonoclonalantibodywasgeneratedagainstglycanandrecognizesgumandtomatoacetylatedmannan.Highlights:ReactswithgumandtomatoacetylatedmannanSuitableforELISA,ImmunolabelingandImmunofluorescenceapplicationsMannanisoneofthemajorconstituentgroupsofhemicelluloseinthewallofhigherplants.ItcompriseslinearorbranchedpolymersderivedfromsugarssuchasD-mannose,D-galactose,andD-glucose.FromthelaboratoryofMichaelG.Hahn,PhD,UniversityofGeorgia.SpecificationsAnti-AcetylatedMannan[CCRC-M169,2C2.D8.E5]Antibody(supernatant)ProductType: AntibodyAntigen: AcetylatedMannanAccessionID: CCRCM169Isotype: IgMkClonality: MonoclonalCloneName: CCRCM169:2C2.D8.E5Reactivity: GumandtomatoImmunogen: Pectin/MeBSASpeciesImmunized: MouseBuffer: CellculturesupernatantTestedApplications: ELISA,Immunolabeling,IFStorage: 1monthat-80CShipped: DryiceDocumentationNotes:Strongreactivitytowardtomatoglucomannan.Weaktomoderatereactivitytoward(1?3)(1?4)-ß-glucan.ThesemonoclonalantibodiesweredevelopedunderthesponsorshipoftheUSNationalScienceFoundation,throughawardnumberDBI-0421683.Theiruseinbiomasscharacterization,studyofbiomassdeconstructionandquantitationwasdevelopedunderthesponsorshipoftheUSDepartmentofEnergythroughawardsDE-PS02-06ER64304andDE-AC05-00OR22725(BioEenergyScienceCenter).AntigenName:AcetylatedMannanAccessionID:CCRCM169ImmunoglobinIsotype:IgMkClonality:MonoclonalCloneName:CCRCM169:2C2.D8.E5Immunogen:Pectin/MeBSASpeciesReactivity:GumandtomatoSpeciesImmunized:MouseFinalBufferConcentration:CellculturesupernatantTestedApplications:ELISA,Immunolabeling,IFStorageTemperature:<1monthat4C,>1monthat-80CShipped:DryIceNotes:Strongreactivitytowardtomatoglucomannan.Weaktomoderatereactivitytoward(1→3)(1→4)-β-glucan.ThesemonoclonalantibodiesweredevelopedunderthesponsorshipoftheUSNationalScienceFoundation,throughawardnumberDBI-0421683.Theiruseinbiomasscharacterization,studyofbiomassdeconstructionandquantitationwasdevelopedunderthesponsorshipoftheUSDepartmentofEnergythroughawardsDE-PS02-06ER64304andDE-AC05-00OR22725(BioEenergyScienceCenter).ReferencesRobinE.Young,HeatherE.McFarlane,MichaelG.Hahn,TamaraL.Western,GeorgeW.HaughnandA.LaceySamuels.2008.AnalysisoftheGolgiApparatusinArABIdopsisSeedCoatCellsduringPolarizedSecretionofPectin-RichMucilage.ThePlantCellJune2008vol.20no.61623-1638.DeMartini,JD,Pattathil,S,Avci,U,Szekalski,K,Mazumder,K,Hahn,M.G.,Wyman,CE:Applicationofmonoclonalantibodiestoinvestigateplantcellwalldeconstructionforbiofuelsproduction.EnergyEnviron.Sci.,2011,4,4332-4339.PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.A.P.deSouza,D.C.C.Leite,S.Pattahil,M.G.Hahn,M.S.Buckeridge.2013.Compositionandstructureofsugarcanecellwallpolysaccharides:Implicationsforsecondgenerationbioethanolproduction.BioenergyResearch6:564-579.J.Puhlmann,E.Bucheli,M.J.Swain,N.Dunning,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1994)Generationofmonoclonalantibodiesagainstplantcellwallpolysaccharides.I.Characterizationofamonoclonalantibodytoaterminalalpha-(1,2)-linkedfucosyl-containingepitope.PlantPhysiol.104:699-710.G.Freshour,R.P.Clay,M.S.Fuller,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1996)Developmentalandtissue-specificstructuralalterationsofthecell-wallpolysaccharidesofArabidopsisthalianaroots.PlantPhysiol.110:1413-1429.G.Freshour,C.P.Bonin,W.-D.Reiter,P.Albersheim,A.G.Darvill,andM.G.Hahn.(2003)Distributionoffucose-containingxyloglucansincellwallsofthemur1mutantofArabidopsisthaliana.PlantPhysiol.131:1602-1612.PattathilS,AvciU,BaldwinD,SwennesAG,McGillJA,PopperZ,BoottenT,AlbertA,DavisRH,ChennareddyC,DongR,O"SheaB,RossiR,LeoffC,FreshourG,NarraR,O"NeilM,YorkWS,HahnMG.(2010)AComprehensiveToolkitofPlantCellWallGlycan-DirectedMonoclonalAntibodies.PlantPhysiol.153:514-525.PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.PattathilS,AvciU,BaldwinD,etal.2010.Acomprehensivetoolkitofplantcellwallglycan-directedmonoclonalantibodies.PlantPhysiology153,514-525.PattathilS,HahnMG,DaleBE,ChundawatSP.Insightsintoplantcellwallstructure,architecture,andintegrityusingglycomeprofilingofnativeandAFEXTM-pre-treatedbiomass.JExpBot.2015Jul;66(14):4279-94.Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.ProviderFromthelaboratoryofMichaelG.Hahn,PhD,UniversityofGeorgia.ReferencesRobinE.Young,HeatherE.McFarlane,MichaelG.Hahn,TamaraL.Western,GeorgeW.HaughnandA.LaceySamuels.2008.AnalysisoftheGolgiApparatusinArabidopsisSeedCoatCellsduringPolarizedSecretionofPectin-RichMucilage.ThePlantCellJune2008vol.20no.61623-1638.DeMartini,JD,Pattathil,S,Avci,U,Szekalski,K,Mazumder,K,Hahn,M.G.,Wyman,CE:Applicationofmonoclonalantibodiestoinvestigateplantcellwalldeconstructionforbiofuelsproduction.EnergyEnviron.Sci.,2011,4,4332-4339.PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.A.P.deSouza,D.C.C.Leite,S.Pattahil,M.G.Hahn,M.S.Buckeridge.2013.Compositionandstructureofsugarcanecellwallpolysaccharides:Implicationsforsecondgenerationbioethanolproduction.BioenergyResearch6:564-579.J.Puhlmann,E.Bucheli,M.J.Swain,N.Dunning,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1994)Generationofmonoclonalantibodiesagainstplantcellwallpolysaccharides.I.Characterizationofamonoclonalantibodytoaterminalalpha-(1,2)-linkedfucosyl-containingepitope.PlantPhysiol.104:699-710.G.Freshour,R.P.Clay,M.S.Fuller,P.Albersheim,A.G.Darvill,andM.G.Hahn.(1996)Developmentalandtissue-specificstructuralalterationsofthecell-wallpolysaccharidesofArabidopsisthalianaroots.PlantPhysiol.110:1413-1429.G.Freshour,C.P.Bonin,W.-D.Reiter,P.Albersheim,A.G.Darvill,andM.G.Hahn.(2003)Distributionoffucose-containingxyloglucansincellwallsofthemur1mutantofArabidopsisthaliana.PlantPhysiol.131:1602-1612.PattathilS,AvciU,BaldwinD,SwennesAG,McGillJA,PopperZ,BoottenT,AlbertA,DavisRH,ChennareddyC,DongR,O"SheaB,RossiR,LeoffC,FreshourG,NarraR,O"NeilM,YorkWS,HahnMG.(2010)AComprehensiveToolkitofPlantCellWallGlycan-DirectedMonoclonalAntibodies.PlantPhysiol.153:514-525.PattathilS,AvciU,MillerJS,HahnMG.2012.Immunologicalapproachestoplantcellwallandbiomasscharacterization:Glycomeprofiling.In:HimmelM(ed)BiomassConversion:MethodsandProtocols.SpringerScience+BusinessMedia,LLC,NewYork,NY,pp61-72.PattathilS,AvciU,BaldwinD,etal.2010.Acomprehensivetoolkitofplantcellwallglycan-directedmonoclonalantibodies.PlantPhysiology153,514-525.PattathilS,HahnMG,DaleBE,ChundawatSP.Insightsintoplantcellwallstructure,architecture,andintegrityusingglycomeprofilingofnativeandAFEXTM-pre-treatedbiomass.JExpBot.2015Jul;66(14):4279-94.Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.

在Kerafast，我们不断为我们难以访问的生物研究材料组合添加新试剂。下面列出了我们的新产品。

 

抗豚鼠GnRH [TF60]抗体

产品描述：该兔多克隆抗体针对对应于豚鼠促性腺激素释放激素（GnRH）的肽产生并识别豚鼠GnRH。

提供者：来自新罕布什尔大学Stacia A. Sower博士实验室。

 

PC3-ML-萤光素酶前列腺癌细胞系

PC3-ML萤光素酶细胞系是表达来自转移性骨的萤光素酶表达的前列腺癌细胞。

来自德雷塞尔大学Mauricio J. Reginato博士的实验室。

 

pFA6A-GFP-ura4MX6质粒

使用pFA6A-GFP-ura4MX6质粒进行基因组GFP标记以在裂殖酵母粟酒裂殖酵母中表达C末端GFP融合蛋白。

Bicyclo[4.2.0]oct-(8)-ene-8-carbonitrile AROMP Polymer

方便制备双环[4.2.0]辛 - （8） - 烯-8-甲腈，其可用于制备醛单体以及其他用于聚合的单体。