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Chemical

Liquid. Supplied as 2 x 1ml vials.

Multi-species

Manufactured by Boston Biochem

Use: 20S Reaction Buffer contains the optimal formulation for the quantitative analysis of constitutive 20S and immuno 20S proteasome activity. This buffer can be used as supplement to the 20S Proteasome Assay Kit or with any individual proteasome enzymes in conjunction with the fluorogenic peptide Suc-Leu-Leu-Val-Tyr-AMC and 20S proteasome activation solution to measure activity. Dilute to 1X in final reaction.

BLUE ICE

+4°C

-20°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -20°C.

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The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 α/β complex, or the PA28 γ complex. The 20S Proteasome is composed of 28 subunits arranged into four stacked rings. The outer rings, containing seven subunits each, are composed of closely-related but non-identical α subunits. The amino-terminal tails of the α subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical β subunits. The amino-terminal tails of six of the β subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention. The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 Activator α.