Details

Description

• Completely nonspecific hydrolyzing after all four bases (1)
• Only available RNase that cleaves the phosphodiester bond of all four bases
• Prefers single-stranded RNA to double-stranded RNA
• Produced from an overexpressing clone in E. coli (2)
• RNase I contains no endonuclease or exonuclease activity toward DNA substrates
• No need for boiling prior to use
• Pure, contains no endonuclease or exonuclease activity toward DNA substrates

Applications

• RNase I degrades RNA to cyclic nucleotide monophosphates leaving a 5-OH and 2-, 3-cyclic monophosphate
• Ideal for ribonuclease protection assays
• Useful for mapping or quantitation of RNA by selective cleavage of single-strand regions

Reagents Supplied

10X RNase I Reaction Buffer

Unit Definition

One unit is the amount of enzymes required to degrade 1µg ofRNA in 30 minutes at 37°Cas detected by TCA precipitation

Storage Buffer

10 mM Tris-HCl (pH 8.0)200 mM NaCl 50% glycerol

Assay Conditions

10 mM Tris-HCl (pH 8.0)100 mM NaCl

Quality Control

All preparations are assayed for contaminating exonuclease and nonspecific endonuclease activities

Storage Conditions

Store at -20°CShipped on Dry Ice

Downloads

MSDS PDF

References

(1) Meador, J. III, and Kennell, D. (1990) Gene 95, 1-7(2) Meador, J. et al. (1990) Eur. J. Biochem. 187, 549