Details

Description

• More potent than competing human placental RNase inhibitors
• RNase-free
• Effective over broad pH range from pH 5.5 to 8.5
• Active over temperature range from 37°C to 70°C
• RNase Inhibitor does not interfere with SP6, T7, or T3 RNA polymerase, AMV or M-MLV reverse transcriptase or Taq DNA polymerase
• RNase Inhibitor increases the time RNA can be safely stored

Unit Definition

One unit is the amount of ribonuclease inhibitor required to inhibit the activity of 5 ng of Ribonuclease A at 25°C (by 50%) Ribonuclease inhibitor activity is determined by the inhibition of RNase A hydrolysis of cyclic 2,3-cytdine monophosphate in a spectrophotometer recording assay

Storage Buffer

20 mM Hepes-KOH (pH 7.5)100 mM KCl0.1 mM EDTA50% (v/v) glycerol

Assay Conditions

0.1 M Tris-acetate (pH 6.5 at 22°C)1 mM EDTA1 mM cyclic 2, 3-cytidine monophosphateReaction volume of 1 ml

Quality Control

All preparations are tested for contaminating endonuclease and exonuclease and nonspecific RNase and single- and double-stranded DNase activites

Storage Contitions

Store at -20°CProduct shipped on dry Ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF