Details
Sph I Source
Streptomyces phaeochromogenesReagents Supplied
10X Sph I Reaction Buffer
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 μlStar Activity
Yes
Reaction Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
10X Sph I Reaction Buffer:50 mM Tris-HCl (pH 7.4 at Room Temperature)6 mM MgCl250 mM NaCl50 mM KCl
Activity in Buffers
Buffer 1:25%Buffer 2:50%Buffer 3: 75%Buffer 4: 25%Buffer 5: 25%*Star activity
Storage Buffer
50 mM Tris‑HCl (pH 7.5 at 4°C)1 mM EDTA100 mM KCl5 mM β-mercaptoethanol0.1% Triton X-100™0.1 mM PMSF500 µg/ml bovine serum albumin50% (v/v) glycerol
Assay Conditions
50 mM Tris‑HCl (pH 7.4 at Room Temperature)6 mM MgCl250 mM NaCl50 mM KCl1 µg lambda DNA
Storage Conditions
Store at -20°CShipped on dry ice
Sph I Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Restriction Endonucleases
