Details

Taq I Source

Thermus aquaticus

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest ΦX-174 DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

Yes

Reaction Temperature

65°C

Heat Inactivation

No

Assay Unit Substrate

ΦX-174 DNA

Reaction Buffer

CMX Buffer 3:50 mM Tris-HCI (pH 7.5 at 37°C)10 mM MgCl₂100 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 25%Buffer 2:75%Buffer 3: 100%Buffer 4: 75%Buffer 5:25%*Star activity

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)300 mM KCl0.1 mM EDTA10 mM β-mercaptoethanol0.1% Triton X-100™500 µg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)10 mM MgCl₂100mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin1 µg ΦX-174 DNAIncubation is at 65°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF - Current LotMSDS PDF- Current Lot

Categories

Restriction Endonucleases