Details
Taq I Source
Thermus aquaticus
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest ΦX-174 DNA in 1 hour in a total reaction volume of 50 μl
Star Activity
Yes
Reaction Temperature
65°C
Heat Inactivation
No
Assay Unit Substrate
ΦX-174 DNA
Reaction Buffer
CMX Buffer 3:50 mM Tris-HCI (pH 7.5 at 37°C)10 mM MgCl2100 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 25%Buffer 2:75%Buffer 3: 100%Buffer 4: 75%Buffer 5:25%*Star activity
Storage Buffer
50 mM Tris-HCl (pH 7.5 at 4°C)300 mM KCl0.1 mM EDTA10 mM β-mercaptoethanol0.1% Triton X-100™500 µg/ml bovine serum albumin50% (v/v) glycerol
Assay Conditions
50 mM Tris‑HCl (pH 7.5 at 37°C)10 mM MgCl2100mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin1 µg ΦX-174 DNAIncubation is at 65°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF - Current LotMSDS PDF- Current Lot
Categories
Restriction Endonucleases