Details
Description
- Mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions and testing replication of mitochondrial DNA
Unit Definition
One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C
Storage Buffer
20 mM Tris-HCl (pH 8.0) 50 mM NaCl50µg/ml PMSF0.05% Triton X-100™10% DMSO5% glycerol
Assay Conditions
25 mM Hepes-KOH (pH 8.0)0.5 mM MnCl210 µg bovine serum albumin2.5 mMβ-mercaptoethanol100 mM NaCl10 µM TTP and 0.3µCi [3H]TTP0.29 mM poly rA:(dT)50Incubation for 15 minutes at 37°Cin a total reaction volume of 15 μl
Quality Control
All preparations are assayed for contaminating endonuclease and exonuclease activities
Storage Conditions
Store at -80°C Long Term, -20°C Short TermAvoid multiple freeze / thaw cyclesShipped on dry ice
Downloads
Certificate of Analysis PDF-Current LotMSDS PDF
