Details
Reagents Supplied
10X Tth DNA Polymerase Reaction Buffer
Source
Thermus thermophilus
Applications
- Ccan reverse transcribe at elevated temperatures
- Minimizes problems with strong secondary structure of RNA
- Results in greater specificity of primer hybridization and extension
- Suitable for high temperature synthesis of DNA
- Synthesizes cDNA from RNA template
- Stable thermophilic DNA polymerase (1)
Unit Definition
One unit is the amount of enzyme required to incorporate 10 nmoles total nucleotide into acid insoluble material at 70°C for 30 minutes.
Concentration
5 - 10 units/µl
Storage Buffer
50 mM Tris HCl (pH 7.5)0.1 mM EDTA5.0 mM dithiothreitol50% (v/v) glycerolStabilizers
Assay Conditions
25 mM Tris-HCl (pH 9.5 at 25°C)50 mM potassium chloride10 mM magnesium chloride1 mM dithiothreitol0.3 µg/μl activated calf thymus DNA1 μg/μl bovine serum albumin0.2 mM of each dCTP, dGTP, dTTP and [α-32P]dATPIncubation is for 10 minutes at 70°Cin a total reaction volume of 50 µl
Storage Conditions
Store at -20°CProduct shipped on dry ice
References
(1) Wang, A.M., Doyle, M.V., and Mark, D.F. (1989) Proc. Nat’l. Acad. Sci. USA 86, 9717-9721
Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Polymerases, Mesophilic DNA Polymerases
