Details

Source

Escherichia coli

Reagents Supplied

5X E. coli Reaction Buffer

Applications

• Catalyzes the formation of a phosphodiester bond between duplex DNA fragments with cohesive ends
• Suitable for high-efficiency cloning of full-length cDNA (1,2)
• Condensation of the 5-phosphoryl group with adjacent 3-hydroxyl group is coupled with the hydrolysis of NAD+

Unit Definition

One unit is the amount of enzyme required to yield 50% ligation of HindIII fragments of lambda DNA in 30 minutes at 16°C in a 20 µl assay mixture containing a DNA terminus concentration of 0.12 µM (300 µg/ml).

Storage Buffer

10 mM Tris-HCl (pH 7.4)50 mM KCl0.1 mM EDTA10 mM ammonium sulfate1 mM dithiothreitol 50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below.

Storage Conditions

Store at -20°C Product shipped on dry ice

Downloads

Certificate of AnalysisLot 2205010 PDF

MSDSMSDS PDF

References

(1) Okayama, H. and Berg, P. (1982) Mol. Cell. Bio. 2, 161-170(2) Gubler, U. and Hoffman, B.J. (1983) Gene 25, 263-269