Details
Nco I Source
Nocardia corallina
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl2100 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1:75%Buffer 2:75%Buffer 3: 75%Buffer 4: 100%Buffer 5: 100%*Star activity
Storage Buffer
50 mM Tris-HCl (pH 7.5 at 4°C)100 mM KCl1 mM EDTA10 mM BME500 µg/ml bovine serum albumin0.1 mM PMSF0.1% Triton X-100™50% (v/v) glycerol
Assay Conditions
20 mM Tris Acetate (pH 7.5 at 37°C)50 mM potassium acetate10 mM magnesium acetate1 mM dithiothreitol100 µg/ml bovine serum albumin1 µg lambda DNA
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF - Current LotMSDS PDF-Current Lot
Categories
Restriction Endonucleases
