Details
Source
Escherichia coli
Description
- Single-stranded specific DNA binding protein (1)
- Helix destabilizing protein (1)
- Ultrapure recombinant protein
Applications
- Reduces formation of secondary DNA structures
- Prevents degradation of single stranded DNA by nucleases
- Prevents inhibition of PCR by template DNA contaminants (2)
- Improves efficiency of DNA amplification by Taq DNA Polymerase (3,4,5,6)
- Replaces Hot Start method by assembling PCR reactions (4,7)
- Stabilizes single-stranded regions of DNA for site-specific mutagenesis
- Aids completion of restriction enzyme digestion
- Improves efficiency of DNA synthesis by T4 DNA Polymerase (8)
- Enhances fidelity of modified T4 DNA Polymerase (8)
Storage Buffer
20 mM Tris-HCl (pH 7.8 at 22°C)300 mM NaCl5 mM β-mercaptoethanol0.05% Igepal0.2 mM EDTA50% (v/v) glycerol
Quality Control
All preperations are assayed for contamination by endonuclease and 3- and 5-exonuclease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis
Storage Conditions
Store at -20°CProduct shipped on Dry Ice
Downloads
Certificate of AnalysisPDF-Current LotMSDS PDF - Current Lot
References
(1) Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp. 61-86(2) Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106(3) Dąbrowski, S., Olszewski, M., Piątek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138(4) Dąbrowski, S. and Kur, J. (1999) Protein Expr. Purif. 16, 96-102(5) Rapley, Mol. Biotech. 2 (1994) 295-298(6) Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079(7) Barski, P., Piechowicz, L., Galinski, J. and Kur, J. (1996) Mol. Cell Probes 10, 471-475
