Details

Source

Aeromonas caviae

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA/Hind III fragments in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

80° for 10 minutes

Assay Unit Substrate

Ad-2 DNA

Reaction Buffer

CMX Buffer 4:20 mM Tris-Acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 75%Buffer 2: 100%Buffer 3: 50%Buffer 4:100%Buffer 5: 75%

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C)1 mM dithiothreitol 50 mM KCl0.1 mM EDTA10 mM MgCl₂200 µg/ml bovine serum albumin 50% (v/v) glycerol

Assay Conditions

20 mM Tris-Acetate (pH 7.5 at 37°C)10 mM Mg Acetate50 mM K Acetate1 mM dithiothreitol100 µg/ml bovine serum albumin 1 µg Ad-2 DNA

Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°CShipped on Dry Ice

Downloads

Certificates of AnalysisLot 2404039 PDF

MSDSMSDS PDF