Details
Source
Moraxella bovis
Reagents Supplied
CMX Buffer 2
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of SV40 DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
No
Incubation Temperature
37°C
Inactivation Temperature
10 minutes at 65°C
Assay Unit Substrate
SV40 DNA
Reaction Buffer
CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albuminNote:Blocked by dam methylation
Activity in Buffers
Buffer 1:75%Buffer 2: 100%Buffer 3: 100%Buffer 4: 75%Buffer 5: 75%
Storage Buffer
10 mM Tris-HCl (pH 7.4 at 4°C)0.1 mM EDTA50 mM KCl1 mM DTT 0.1% Triton X-100™500 µg/ml bovine serum albumin50% (v/v) glycerol
Assay Conditions
100µg/ml BSA10 mM Tris‑HCl (pH 7.5 at 37°C) 50 mM NaCl10 mM MgCl21 mM dithiothreitol1 µg SV40 DNAIncubation is at 37°C for 1 hour in a reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Restriction Endonucleases
