Details

Bal I Source

Brevibacterium albidum

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 30 μl

Heat Inactivation

65°C for 10 minutes

Incubation Temperature

37°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:20 mM Tris-acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100 μg/ml bovine serum albumin

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 22°C)1 mM dithiothreitol50 mM KCl0.1 mM EDTA200 μg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

20 mM Tris-acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100 μg/ml bovine serum albumin1 μg of lambda DNAIncubation for 1 hour at 37°C in a total reaction volume of 30 μl

Quality Control

All preparations are assayed for contaminating endonuclease, 3-exonuclease, and nonspecific single- and double-stranded DNase activities

Storage Conditions

Store at -20°CShipped on Dry Ice