Details
Source
Haemophilus aegyptius
Reagents Supplied
CMX Buffer 2
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
80°C for 10 minutes
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 2:100%*Star activity
Storage Buffer
50 mM Tris‑HCl (pH 7.5 at 4°C) 100 mM KCl0.5 mM dithiothreitol500 µg/ml bovine serum albumin 50% (w/v) glycerol
Assay Conditions
10 mM Tris-HCl (pH 7.5 at 37°C)10 mMMgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin1 µg lambda DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of AnalysisPDF-Current LotMSDS PDF-Current Lot
