Immunohistochemistry ServicesWork with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies. TCR Screening ServicesTest your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their \"Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use\".Customer.Support@lsbio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues. Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development. Worldwide Distributors List - To find your local distributor if you\'re not within the United States.order histories, retained contact details for faster checkout, review submissions, and special promotions.order histories, retained contact details for faster checkout, review submissions, and special promotions. International 206-374-1102 For Research Use Only Description E2 antibody LS-B4677 is an unconjugated rabbit polyclonal antibody to E2 (DBT) (aa35-84) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Synonyms DBT | BCKAD E2 subunit | BCKAD-E2 | BCKADE2 | Dihydrolipoyl transacylase | E2 | E2B | BCATE2 Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity) Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. Immunogen Synthetic peptide located between aa35-84 of human DBT (P11182, NP_001909). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Zebrafish, Beetle (92%); Trout, Drosophila (91%); Stickleback, Sea squirt (85%). LS-E18663 - Lyophilized - 100 g - $145.00 Immunizing peptide used to generate LS-B4677. Useful for pre-absorption and neutralization of the antibody s antigen binding site. Usage Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. Storage Short term: Store at 2-8 C. Long term: Aliquot and store at -20 C. Avoid freeze/thaw cycles. This antibody carries the LSBio 100% Guarantee. LSBio Guarantee Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested. Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested. Anti-DBT / E2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested. Anti-DBT / E2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested. Anti-DBT / E2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested. Anti-DBT / E2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested. IHC-plus™ DBT / E2 Antibody (aa35-84) for IHC, WB/Western LS-B4677 has an LSBio Rating of(4.0) Rating Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay