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...Claudin 1 (Tyr210) Antibody - AF8256 - affinity_biosciences
PCI – Protein C Inhibitor 多克隆抗体 – HRP 共轭抗体

Affinity 的 PCI – Protein C Inhibitor 多克隆抗体 – HRP 共轭是从与辣根过氧化物酶共轭的抗血清中纯化的完整 IgG。 IgG-HRP 以含有 50% 甘油 (v/v) 的缓冲稳定剂溶液形式提供。通过分光光度法测定,该缀合物的 Rz 比(Reinheitszahl,A403/A280)≥0.25。总蛋白为 0.2 mg。

宿主动物:山羊

应用:该抗体通常用作免疫测定和免疫印迹等应用中的标记一抗

查看物种交叉反应性

规格储存和保质期储存

-10°C 至 -20°C

类型

冷冻

有效期

请查询

可用格式Cat #FormatGAPCI-HRP

1 x 0.2 mg

仅供研究使用。文件产品插页更多信息安全数据表更多信息相关产品PCI - 蛋白 C 抑制剂多克隆抗体 - 亲和纯化

GAPCI-AP – 山羊抗人 PCI – 蛋白 C 抑制剂,亲和纯化 IgG(0.5 毫克小瓶)

蛋白 C 抑制剂缺陷血浆- 冷冻

PCI-DP – 冷冻蛋白 C 抑制剂缺陷血浆 – 1.0 mL 或散装数量

纤溶酶原多克隆抗体 - 亲和纯化 - HRP 缀合抗体

Affinity 的纤溶酶原多克隆抗体 - 亲和纯化 - HRP 缀合是我们最高水平的辣根过氧化物酶缀合纤溶酶原 (Pg) 抗体。在抗原亲和纯化过程中,IgG 已消除任何非特异性免疫球蛋白部分,这增强了剩余免疫球蛋白对靶抗原的特异性。结果是纯度非常高的产品,其滴度明显高于完整或纯化的 IgG。我们的纤溶酶原多克隆抗体 - 亲和纯化 - HRP 缀合在含有 50% 甘油 (v/v) 的缓冲稳定剂溶液中提供。

宿主动物:山羊

应用:该抗体是通常用作免疫测定和免疫印迹等应用中的标记一抗。

查看物种交叉反应性

规格存储和保质期< p>-10°C 至 -20°C类型

冷冻

有效期

请查询

可用格式Cat #FormatGA​​PG-APHRP

1 x 0.1 mg仅供研究使用。文档产品插页更多信息安全数据表更多信息相关产品纤溶酶原缺乏血浆 - 冷冻

PG-DP – 冷冻纤溶酶原缺乏血浆 – 1.0 mL 或散装

纤溶酶原缺乏血浆 - 冻干

PG-LDP –冻干纤溶酶原缺陷血浆 – 1.0 mL

纤溶酶原配对抗体套装

PG-EIA – 纤溶酶原配对抗体套装 – 5 板套装

纤溶酶原多克隆抗体 - 亲和纯化 - 山羊

GAPG-AP – 山羊抗人纤溶酶原,亲和纯化 IgG(0.5 毫克小瓶)

纤溶酶原多克隆抗体 - 亲和纯化 - 羊

SAPG-AP – Sheep 抗人纤溶酶原,亲和纯化 IgG(0.5 毫克小瓶)

纤溶酶原多克隆抗体 - 山羊

GAPG-IG – 山羊抗人纤溶酶原,纯化 IgG(5 毫克小瓶)

纤溶酶原多克隆抗体 -绵羊

SAPG-IG – 绵羊抗人纤溶酶原,纯化 IgG(5.0 毫克小瓶)

纤溶酶原多克隆抗体 – 山羊抗体

Affinity 纤溶酶原多克隆抗体 – 山羊是我们纤溶酶原 (Pg) 抗体家族的基础水平。 IgG 的纯度通常为 90%,以含有 50% 甘油 (v/v) 的 HEPES 缓冲盐水溶液形式提供。滴度基本上与起始抗血清相同,并且每个小瓶通常含有从一毫升抗血清中回收的 IgG 量。这种纤溶酶原多克隆抗体 - 山羊通常用于免疫沉淀、免疫电泳、免疫耗竭和活性中和测定等应用。

宿主动物:山羊

查看物种交叉反应性

规格储存和保质期储存

-10°C 至 -20°C

有效期

请查询

可用格式sCat #FormatGA​​PG-IG

1 x 5.0 mg

仅供研究使用。文件产品插页更多信息安全数据表更多信息相关产品纤溶酶原缺乏血浆 - 冷冻

PG-DP – 冷冻纤溶酶原缺乏血浆 – 1.0 mL 或散装

纤溶酶原缺乏血浆 - 冻干

PG-LDP – 冻干纤溶酶原缺乏血浆 – 1.0 mL

纤溶酶原配对抗体套装

PG-EIA – 纤溶酶原配对抗体套装 – 5 板套装

纤溶酶原多克隆抗体 - 亲和纯化 - 山羊

GAPG-AP – 山羊抗人纤溶酶原,亲和纯化IgG(0.5 毫克小瓶)

纤溶酶原多克隆抗体 - 亲和纯化 - HRP 偶联

GAPG-APHRP – 山羊抗人纤溶酶原,亲和纯化,过氧化物酶偶联。 IgG(0.1 毫克小瓶)

纤溶酶原多克隆抗体 - 亲和纯化 - 绵羊

SAPG-AP – 绵羊抗人纤溶酶原,亲和纯化 IgG(0.5 毫克小瓶)

纤溶酶原多克隆抗体 - 绵羊

SAPG-IG – 绵羊抗人纤溶酶原,纯化 IgG(5.0 毫克小瓶)

Prediction Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(86%), Xenopus(83%) Alternative Names:Expand▼ Claudin-1; Claudin1; CLD 1; CLD1; CLD1_HUMAN; CLDN 1; Cldn1; ILVASC; SEMP 1; SEMP1; Senescence associated epithelial membrane protein 1; Senescence associated epithelial membrane protein; Senescence-associated epithelial membrane protein; Applications: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000 *The optimal dilutions should be determined by the end user. Reactivity: Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(86%), Xenopus(83%) Source: Rabbit Clonality: Polyclonal Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Specificity: Phospho-Claudin 1 (Tyr210) Antibody detects endogenous levels of Claudin 1 only when phosphorylated at Tyr210. RRID: AB_2840318 Please cite this product as: Affinity Biosciences Cat# AF8256, RRID:AB_2840318. Format: Liquid Concentration: 1mg/ml Storage Condition and Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Immunogen: A synthesized peptide derived from human Claudin 1 around the phosphorylation site of Tyr210. Uniprot: Visit The Human Protein Atlas Gene ID: Gene Name: CLDN1 Molecular Weight: Observed Mol.Wt.: 22kD. Predicted Mol.Wt.: 23kDa(Calculated).. Subcellular Location: Cell junction, tight junction. Cell membrane. Tissue Specificity: O95832 CLD1_HUMAN:Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. Function: Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus. Subcellular Location: Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein. Basolateral cell membrane. Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane. Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte Tissue Specificity: Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. Subunit Structure: Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ (By similarity). Interacts with OCLN, CLDN4, CLDN6 and CLDN9. Interacts with CD81.(Microbial infection) Interacts with hepatitis c virus E1 and E2 proteins.(Microbial infection) Interacts with dengue virus small envelope protein M. Similarity: Belongs to the claudin family. ELISA analysis of AF8256 showing specificity to Phospho-Claudin 1 (Tyr210) Blocking Peptide peptide. Peptides concentration: 1ug/ml. P-peptide: phospho-peptide; N-peptide: non-phospho-peptide. Information:(Personal information is only used for customer service and will not be disclosed) Name AF8256-BP (Blocking peptide available as AF8256-BP) Price/Size : $350/1mg. Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg). Function : Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody. Format and storage : Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Precautions : This product is for research use only. Not for use in diagnostic or therapeutic procedures. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.