Enzolifesciences/Cytochrome P450 2E1 polyclonal antibody/BML-CR3271-0025/25µl
Product Specification:
| Alternative Name: | CYP2E1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the C-terminus of hepatic human, rat, rabbit, and mouse CYP2E1. |
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| UniProt ID: | P05181 (human), P05181 (mouse), P05182 (rat), P08682 (rabbit) |
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| Species reactivity: | Human, Mouse, Rat |
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| Applications: | IHC (FS), IHC (PS), WB |
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| Recommended Dilutions/Conditions: | The antiserum detects CYP2E1 in rat liver microsomal preparations and in rat brain by Western blotting (~57kDa) and in immunohistochemical applications at a dilution of 1:1000 or greater when using sensitive detection methods. Such as enhanced chemiluminescence (ECL; Amersham) for Western blotting applications, and ABC-Elite (Vector Laboratories) for immunohistochemistry. The use of 2-4% neutral-buffered formaldehyde or methacarn fixation is recommended. Frozen sections are recommended, but dewaxed tissue sections have also been used satisfactorily. Antigen retrieval techniques may enhance signal generation.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~57kDa by Western blot. |
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| Blocking Peptide: | For Blocking peptide see Prod. No. BML-CP0270. |
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| Formulation: | Rabbit antiserum. Contains no preservatives. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Cytochrome P-450 2E1(CYP2E1) is principally found in the liver, and is ethanol-inducible. It catalyzes many biotransformations. In rat brain CYP2E1 is localized to cells within the substantia nigra and in striatial blood vessels where a possible role in the pathology of oxidative stress, believed to underlie nigral degeneration has been proposed. Recently, increased expression of human CYP2E1 mRNA, along with that of several other CYP genes, has been found in the uterine endometrium of patients treated with tamoxifen. |
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