Enzolifesciences/Chk2 monoclonal antibody (DCS-270)/ADI-KAM-CC112-E/100µg
Product Specification:
| Alternative Name: | Checkpoint kinase 2 |
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| Clone: | DCS-270 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant human Chk2. |
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| UniProt ID: | O96017 |
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| Species reactivity: | Human |
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| Applications: | IHC, IP, WB |
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| Recommended Dilutions/Conditions: | Immunohistochemistry (1-5µg/ml)Immunoprecipitation (3µg/200-300µl)Western Blot (0.2µg/ml, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The DNA damage checkpoint is a signal transduction pathway that has the ability to delay entry into mitosis. When DNA is damaged, the Check point kinases (CHKs) are known to act downstream of ATM to elicit appropriate responses such as cell cycle arrest. Chk1, for instance, is activated by ATM phosphorylation of serines 123, 178, 278, and 292 of the S phase-promoting cdc25A phosphatase and accelerates IR (ionizing radiation)induced degradation of cdc25A. |
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