| Alternative Name: | DXS1357E, B cell receptor-associated protein BAP31, BCR-associated protein BAP31, p28 BAP31, CDM protein, 6C6-AG tumor-associated antigen, BCAP31 |
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| Clone: | CC-1 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Solubilized membranes of the IMR-32 human neuroblastoma cell line. |
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| UniProt ID: | P51572 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | HumanBovine, Hamster, Primate |
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| Specificity: | Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, bovine and hamster BAP31. |
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| Crossreactivity: | Does not recognize mouse and rat BAP31. |
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| Applications: | ELISA, ICC, IHC (FS), IP, WB |
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| Recommended Dilutions/Conditions: | ELISA (capture)Immunocytochemistry (1:1,000-1:10,000)Immunohistochemistry (frozen sections, 1:1,000-1:10,000)Western Blot (1:1,000-1:6,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
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| Application Notes: | Works on endogenous in ICC, IHC and WB. |
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| Formulation: | Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca2+-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2. |
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