Enzolifesciences/Aryl hydrocarbon receptor monoclonal antibody (RPT1)/ALX-804-421-R100/100µl
Product Specification:
| Alternative Name: | AhR |
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| Clone: | RPT1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to aa 12-17 (R12KRRKP17)and aa 22-31 (V22KPIPAEGIK31)of mouse AhR (aryl hydrocarbon receptor). |
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| UniProt ID: | P30561 |
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| Species reactivity: | Human, Mouse, Rat |
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| Applications: | IF, IHC (PS), IP, WB |
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| Recommended Dilutions/Conditions: | Immunofluorescence (1:100-1:1,000)Immunohistochemistry (paraffin sections, 1:10-1:100)Western Blot (1:1500-1:2,000). Suggested dilutions/conditions may not be available for all applications. Optimal should be determined individually for each application. |
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| Application Notes: | Detects a band of ~95kDa by Western blot. |
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| Formulation: | Liquid. Ascites containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1. |
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