Description:

Short Description: Transferrin (TX) is a metal-combining protein that reversibly binds to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participate in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, transferrin is synthesized in the liver, but unlike haptoglobin transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system. This ELISA kit can be used to measure transferrin in serum, tissue extracts and other biological fluids.

Additional Information:

Name Related Product Names Gene Symbol Gene Fullname Gene id Alias Symbols Protein Name Description of Target Swissprot ID Species Reactivity Assay Info Sensitivity Kit Range Homology NCBI Acc Number Standard Range Sample Type Product Format Specimen/Volumes Swiss Prot Number Applications Reconstitution and Storage Reactivity Kit Size Kit Principle Kit Component Kit Duration Storage Lead Time Intended Use Additional Information ::

Rat Transferrin ELISA Kit

Rat Transferrin ELISA Kit; Transferrin; Siderophilin; Beta-1-metal-binding globulin Rat Transferrintrfe

TF

transferrin

24825

Serotransferrin, transferrin, Beta-1 metal-binding globulin, TF, Siderophilin

Serotransferrin

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage a

P12346

Rat

Assay Methodology: Quantitative Sandwich ELISA

1.153 ng/ml

6.25 ng/ml - 400 ng/ml

Rat

NP_001054.1

Refer To Manual

Serum, plasma and other biological fluids

In Solution

Refer To Manual

P02787

ELISA

1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-TX conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-RAT TRANSFERRIN ELISA MICRO PLATEAnti-Rat TX coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. RAT TRANSFERRIN CALIBRATORThe lyophilized Rat Transferrin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.

Rat

96 well

Genway Biotech Transferrin ELISA Kit (Rat) (GWB-2C5ED4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serotransferrin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serotransferrin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serotransferrin captured in the well.

ComponentAmount Anti-Rat Transferrin_x000D_ Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Rat Transferrin_x000D_ Calibrator 1

40 Minutes

Refer to kit instructions for component storage

Domestic: within 1 week deliveryInternational: 1 week

Research Use Only

Intended Use: The Transferrin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in biological samples of rats.

Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Rat Transferrin antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-RAT TRANSFERRIN ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat TX.7. RAT TRANSFERRIN CALIBRATOROne vial containing a lyophilized Rat Transferrin calibrator.