Description:
Short Description: In this assay the HAPTOGLOBIN present in serum sample reacts with the anti-HAPTOGLOBIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.
Additional Information:
Name Rat Haptoglobin ELISA Kit |
Related Product Names HP2-ALPHA; Rat Haptoglobin ELISA Kit; N/A Rat HaptoglobinHP |
Gene Symbol HP |
Gene Fullname haptoglobin |
Gene id 24464 |
Alias Symbols Haptoglobin, HP, Zonulin |
Protein Name Haptoglobin |
Description of Target As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also a |
Swissprot ID P06866 |
Species Reactivity Rat |
Assay Info Assay Methodology: Quantitative Sandwich ELISA |
Sensitivity 1.047 ng/ml |
Kit Range 1.95 ng/ml - 125 ng/ml |
Homology Rat |
NCBI Acc Number NP_001119574.1 |
Standard Range Refer To Manual |
Sample Type Serum, plasma and other biological fluids |
Product Format In Solution |
Specimen/Volumes Refer To Manual |
Swiss Prot Number P00738 |
Applications ELISA |
Reconstitution and Storage 1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-Hp conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-RAT HAPTOGLOBIN ELISA MICRO PLATEAnti-Rat Hp coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. RAT HAPTOGLOBIN CALIBRATORThe lyophilized Rat Haptoglobin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
Reactivity Rat |
Kit Size 96 well |
Kit Principle Genway Biotech Haptoglobin ELISA Kit (Rat) (GWB-557416) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well. |
Kit Component ComponentAmount Anti-Rat Haptoglobin Microplate | 96 Wells (12 x 8 Well Strips) | 20X Diluent Concentrate | 50 mL | 20X Wash Solution | 50 mL | 100X Enzyme-Antibody Conjugate | 150 uL | Chromogen-Substrate Solution | 12 mL | Stop Solution | 12 mL | Rat Haptoglobin Calibrator | 1 |
|
Kit Duration 40 Minutes |
Storage Refer to kit instructions for component storage |
Lead Time Domestic: within 1 week deliveryInternational: 1 week |
Intended Use Research Use Only |
Additional Information Intended Use: The Haptoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in biological samples of rats |
:: Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Rat Haptoglobin antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-RAT HAPTOGLOBIN ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat Hp.7. RAT HAPTOGLOBIN CALIBRATOROne vial containing a lyophilized Rat Haptoglobin calibrator. |