GenWay/Human Plasminogen ELISA Kit/GWB-91D15C/96 well
Additional Information:
Name | Human Plasminogen ELISA Kit |
Related Product Names | Human Plasminogen ELISA Kit |
Gene Symbol | PLG |
Gene Fullname | plasminogen |
Gene id | 5340 |
Alias Symbols | Plasminogen, PLG, Plasmin, Plasmin light chain B |
Protein Name | Plasminogen |
Description of Target | Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It |
Swissprot ID | P00747 |
Species Reactivity | Human |
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Sensitivity | 2.114 ng/ml |
Kit Range | 6.25 ng/ml - 200 ng/ml |
Homology | Human |
Standard Range | Refer To Manual |
Sample Type | Serum, plasma and other biological fluids |
Product Format | In Solution |
Specimen/Volumes | Refer To Manual |
Applications | ELISA |
Reconstitution and Storage | 1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-PMG conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-HUMAN PLASMINOGEN ELISA MICRO PLATEAnti-Human PMG coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. HUMAN PLASMINOGEN CALIBRATORThe lyophilized Human Plasminogen calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
Reactivity | Human |
Kit Size | 96 well |
Kit Principle | Genway Biotech Plasminogen ELISA Kit (Human) (GWB-91D15C) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Plasminogen has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Plasminogen - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plasminogen captured in the well. |
Kit Component ComponentAmount | Anti-Human Plasminogen Microplate | 96 Wells (12 x 8 Well Strips) | | 20X Diluent Concentrate | 50 mL | | 20X Wash Solution | 50 mL | | 100X Enzyme-Antibody Conjugate | 150 uL | | Chromogen-Substrate Solution | 12 mL | | Stop Solution | 12 mL | | Human Plasminogen Calibrator | 1 |
|
Kit Duration | 130 Minutes |
Storage | Refer to kit instructions for component storage |
Lead Time | Domestic: within 1 week deliveryInternational: 1 week |
Intended Use | Research Use Only |
Additional Information | Intended Use: The Plasminogen test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Plasminogen in biological samples of human. |
:: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Human Plasminogen antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-HUMAN PLASMINOGEN ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human PMG.7. HUMAN PLASMINOGEN CALIBRATOROne vial containing a lyophilized Human Plasminogen calibrator. |
