.product-side h2 span {width: 50%;}.product-side h2 span.hover {}Related ProductsMolarity CalculatorDilution Calculator Bestatin hydrochlorideInhibitor of aminopeptidase N (APN)/CD13 and aminopeptidase B.Catalog No.A8621SizePriceStockQty5mg$50.00In stock 10mg$70.00In stock 25mg$120.00In stock 100mg$340.00In stock Tel: +1-832-696-8203Email: sales@apexbt.comWorldwide Distributors Add to CartBulk InquiryFree samples Check OutSample solution is provided at 25 µL, 10mM.Publications citing ApexBio ProductsNature.2017 Jan 19;541(7637):417-420.Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control Quality Control & MSDS View current batch: 321 Purity = 98.00% COA (Certificate Of Analysis) MSDS (Material Safety Data Sheet) Datasheet Purity = 98.95% COA (Certificate Of Analysis) HPLC NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Purity = 98.09% COA (Certificate Of Analysis) HPLC NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Chemical structure ProtocolCell experiment [1]:Cell linesD. discoideum cells, human umbilical vein endothelial cellsPreparation methodSoluble in DMSO. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.Reacting condition600 μM, 48 hApplicationsBestatin (600 μM)-treated cells progressed slower through the cell cycle due to decreased rate of cell growth and the frequency of cell division. Bestatin inhibited the frequency of mitosis and the inherent multinuclearity in D. discoideum cells, and was not cytotoxic to D. discoideum cells at 0-600 μM. Bestatin inhibited aminopeptidase activity in lysates of PsaA-GFP- and GFP-expressing cells by 69.39% ± 10.5% and 39.93% ± 18.7% of control, respectively. Bestatin (1-100 μg/ml) dose-dependently inhibited the Ala-MCA-hydrolysing activity of HUVECs. Bestatin inhibited the tube-like formation of HUVECs.Animal experiment [2]:Animal modelsInbred 6-week-old female C57BL/6 mice bearing B16-BL6 melanoma xenograftsDosage formOral administration, 100-200 mg/kg/day; Intraperitoneal injection, 50-100 mg/kg/dayApplicationIn a mouse dorsal air sac assay, oral administration of bestatin (100-200 mg/kg/day) significantly inhibited melanoma cell-induced angiogenesis. After the orthotopic implantation of B16-BL6 melanoma cells into mice, bestatin administration (50-100 mg/kg/day, i.p.) reduced the number of vessels oriented towards the established primary tumor mass on the dorsal side of mice.Other notesPlease test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.References:[1]. Poloz Y, Catalano A, O"Day D H. Bestatin inhibits cell growth, cell division, and spore cell differentiation in Dictyostelium discoideum[J]. Eukaryotic cell, 2012, 11(4): 545-557.[2]. Aozuka Y, Koizumi K, Saitoh Y, et al. Anti-tumor angiogenesis effect of aminopeptidase inhibitor bestatin against B16-BL6 melanoma cells orthotopically implanted into syngeneic mice[J]. Cancer letters, 2004, 216(1): 35-42.Bestatin hydrochloride Dilution CalculatorConcentration (start)xVolume (start)=Concentration (final)xVolume (final) femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter femtomolar picomolar nanomolar micromolar millimolar molar microliter milliliter liter C1V1C2V2calculateBestatin hydrochloride Molarity CalculatorMass=ConcentrationxVolumexMW* picograms nanograms micrograms milligrams grams kilograms femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter g/molcalculate Chemical Properties Cas No. 65391-42-6SDF Download SDF Synonyms N/A Chemical Name (2S)-2-[[(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl]amino]-4-methylpentanoic acid;hydrochloride Canonical SMILES CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O.Cl Formula C16H25ClN2O4 M.Wt 344.83 Solubility ≥125 mg/mL in DMSO, ≥34.2 mg/mL in H2O, ≥68 mg/mL in EtOH Storage Store at -20°CShipping ConditionEvaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon requestGeneral tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. BackgroundBestatin hydrochloride, also known as Ubenimex, is an inhibitor of aminopeptidase B and N (APN)/CD13 [1].Bestatin, an antibiotic of microbial origin, is a potent inhibitor of some aminopeptidases which can be administered to cultured cells, intact animals and humans with low toxicity. It has been used as an useful tool to assess the physiological role of certain mammalian exopeptidases in the regulation of the immune system, the growth of tumors and their invasion of surrounding tissues, and the degradation of cellular proteins [2].In vivo: In a mouse dorsal air sac assay, oral administration of bestatin (100-200 mg/kg/day) showed a significant inhibitory activity against the melanoma cell-induced angiogenesis. Bestatin also inhibited the tube-like formation of human umbilical vein endothelial cells (HUVECs). Furthermore, after the orthotopic implantation of B16-BL6 melanoma cells into mice, bestatin administration (50-100 mg/kg/day, i.p) reduced the number of vessels oriented towards the established primary tumor mass on the dorsal side of mice [1].References:[1].  Aozuka Y1, Koizumi K, Saitoh Y, Ueda Y, Sakurai H, Saiki I. Anti-tumor angiogenesis effect of aminopeptidase inhibitor bestatin against B16-BL6 melanoma cells orthotopically implanted into syngeneic mice. Cancer Lett. 2004 Dec 8;216(1):35-42.[2].  Scornik OA1,Botbol V.Bestatin as an experimental tool in mammals. Curr Drug Metab.2001 Mar;2(1):67-85.