.product-side h2 span {width: 50%;}.product-side h2 span.hover {}Related ProductsMDL 28170PD 150606Acetyl-Calpastatin (184-210) (human)Calpain Inhibitor I, ALLNE-64PD 151746Molarity CalculatorDilution Calculator Calpain Inhibitor II, ALLMCalpain inhibitorCatalog No.A2603SizePriceStockQty10mM (in 1mL DMSO)$80.00In stock 5mg$50.00In stock 25mg$200.00In stock Tel: +1-832-696-8203Email: sales@apexbt.comWorldwide Distributors Add to CartBulk InquiryFree samples Check OutSample solution is provided at 25 µL, 10mM.Product Citations1.Li W, Liu BD, et al. "Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells." Medicina (Kaunas). 2018 May 30;54(3). pii: E39.PMID:303442702.Chen Y, Yu Y, et al. "Bradykinin promotesmigration and invasion of hepatocellular carcinoma cells through TRPM7 and MMP2." Exp Cell Res. 2016 Sep 29.PMID:27693494Related Compound Libraries DiscoveryProbe™ Bioactive Compound LibraryDiscoveryProbe™ Protease Inhibitor LibraryQuality Control Quality Control & MSDS View current batch: 54321 Purity = 98.00% COA (Certificate Of Analysis) Request NMR (NMR of exclusive products available upon request.) MSDS (Material Safety Data Sheet) Datasheet Purity = 98.00% COA (Certificate Of Analysis) NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Purity = 98.00% COA (Certificate Of Analysis) NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Purity = 98.00% COA (Certificate Of Analysis) NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Purity = 98.00% COA (Certificate Of Analysis) NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Chemical structure Related Biological Data Related Biological Data Biological ActivityDescriptionCalpain Inhibitor II, ALLM is a cell-permeable inhibitor of calpain I, calpain II, cathepsin L and cathepsin B with Ki values of 120 nM, 230 nM, 0.6 nM and 100 nM, respectively.Targetscalpain Icalpain IIcathepsin Lcathepsin BIC50120 nM (Ki)230 nM (Ki)0.6 nM (Ki)100 nM (Ki)ProtocolCell experiment [1]:Cell linesAcute lymphoblastic leukemia (ALL) cell lines (ALL-1, RS4;11, and JURKAT) and non-Hodgkin’s lymphoma (NHL) cell lines (RAMOS and DAUDI)Preparation methodThe solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.Reaction Conditions50 or 100 μM; 24 hrsApplicationsAt the dose of 50 or 100 μM, Calpain inhibitor II induced apoptosis in ALL (ALL-1, RS4;11, and JURKAT) and NHL (RAMOS and DAUDI) cell lines. Additionally, studies had shown that neither BTK nor LYN were required for Calpain inhibitor II induced apoptosis. Calpain inhibition with Calpain inhibitor II had been demonstrated to activate an apoptosis-promoting caspase system.References:[1]. Zhu DM1, Uckun FM. Calpain inhibitor II induces caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin"s lymphoma cells as well as some solid tumor cells. Clin Cancer Res. 2000 Jun;6(6):2456-63.Calpain Inhibitor II, ALLM Dilution CalculatorConcentration (start)xVolume (start)=Concentration (final)xVolume (final) femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter femtomolar picomolar nanomolar micromolar millimolar molar microliter milliliter liter C1V1C2V2calculateCalpain Inhibitor II, ALLM Molarity CalculatorMass=ConcentrationxVolumexMW* picograms nanograms micrograms milligrams grams kilograms femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter g/molcalculate Chemical Properties Cas No. 136632-32-1SDF Download SDF Synonyms N-Acetyl-Leu-Leu-Methional Chemical Name 2-acetamido-4-methyl-N-[4-methyl-1-[(4-methylsulfanyl-1-oxobutan-2-yl)amino]-1-oxopentan-2-yl]pentanamide Canonical SMILES CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCSC)C=O)NC(=O)C Formula C19H35N3O4S M.Wt 401.57 Solubility ≥14.85mg/mL in DMSO Storage Store at -20°CPhysical AppearanceA solidShipping ConditionEvaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon requestGeneral tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. BackgroundCalpain inhibitor II (CPI-2) is a cell-permeable inhibitor of calpain I, calpan II, cathepsin L and cathepsin B. Calpain inhibitor II 2 at 50 or 100 μM has shown to induce apoptosis in (acute lymphoblastic leukemia) ALL (ALL-1, RS4;11, and JURKAT) and (non-Hodgkin’s lymphoma) NHL (RAMOS and DAUDI) cell lines, as measured by MC540 single fluorescence. Additionally, studies have shown that neither BTK nor LYN were required for calpain inhibitor II induced apoptosis. Calpain inhibition with calpain inhibitor II has been demonstrated to induce apoptosis-promoting caspase system [1]. Unlike calpain inhibitor I, calpain inhibitor II cannot inhibit NFkB and sensitize DLD1-TRAIL/R cells to the TRAIL protein [2].References:[1] Zhu DM1, Uckun FM. Calpain inhibitor II induces caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin"s lymphoma cells as well as some solid tumor cells. Clin Cancer Res. 2000 Jun;6(6):2456-63.[2] Zhu H1, Zhang L, Huang X, Davis JJ, Jacob DA, Teraishi F, Chiao P, Fang B.Overcoming acquired resistance to TRAIL by chemotherapeutic agents and calpain inhibitor I through distinct mechanisms. Mol Ther. 2004 May;9(5):666-73.