.product-side h2 span {width: 50%;}.product-side h2 span.hover {}Related ProductsMolarity CalculatorDilution Calculator GeldanamycinHsp90 inhibitor,potent and specificCatalog No.A4060SizePriceStockQty10mM (in 1mL DMSO)$160.00In stock 5mg$90.00In stock 10mg$140.00In stock 50mg$410.00In stock 100mg$650.00In stock Tel: +1-832-696-8203Email: sales@apexbt.comWorldwide Distributors Add to CartBulk InquiryFree samples Check OutSample solution is provided at 25 µL, 10mM.Product Citations1. Deng X, Song L, et al. "HAX-1 Protects Glioblastoma Cells from Apoptosis through the Akt1 Pathway." Front Cell Neurosci. 2017 Dec 21;11:420.PMID:293118402. Guo XB, Deng X, et al. "Hematopoietic Substrate-1-Associated Protein X-1 Regulates the Proliferation and Apoptosis of Endothelial Progenitor Cells Through Akt Pathway Modulation." Stem Cells. 2018 Mar;36(3):406-419.PMID:29139175Quality Control Quality Control & MSDS View current batch: 1 Purity = 97.01% COA (Certificate Of Analysis) HPLCMS (Mass Spectrometry)MSDS (Material Safety Data Sheet) Datasheet Chemical structure Related Biological Data Related Biological Data ProtocolKinase experiment [1]:Isothermal titration calorimetry (ITC) of nucelotide bindingThe titration experiments were performed using the MSC system. In each experiment, 16 aliquots of 15 μL of Geldanamycin (300 μM in 1% DMSO) were injected into 1.3 mL of protein (31 μM in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA) at 25°C, and the resulting data were fit after subtracting the heats of dilution. Heats of dilution were determined in separate experiments from addition of Geldanamycin into buffer and buffer into protein. No evidence for binding of DMSO in the nucleotide binding site was observed. Titration data were fit using a nonlinear least-squares curve-fitting algorithm with three floating variables: stoichiometry, binding constant (Kb = 1/Kd), and change of enthalpy of interaction (ΔH°). The dissociation constant estimated for Geldanamycin binding to intact yeast Hsp90 was 1.22 μM, and for binding to Hsp90 N-terminal domain was 0.78 μM. No meaningful heat was observed with binding to the C-terminal fragment.Cell experiment [2]:Cell linesA2780 cellsPreparation methodThe solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.Reaction Conditions0.001 ~ 10 μM; 3 hrsApplicationsIn human ovarian cell line A2780, Geldanamycin caused a dose-dependent G2 arrest and reversible inhibiton of entry into the S phase.Animal experiment [3]:Animal modelsMice bearing FRE/erbB-2 tumorsDosage form50, 100, 200 and 400 mg/kg; i.p.; b.i.d., for 5 daysApplicationsIn mice bearing FRE/erbB-2 tumors, Geldanamycin (50 mg/kg) shows 30% inhibition on p185-associated phosphotyrosine levels.Other notesPlease test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.References:[1]. Roe SM, Prodromou C, O"Brien R, Ladbury JE, Piper PW, Pearl LH. Structural basis for inhibition of the Hsp90 molecular chaperone by the antitumor antibiotics radicicol and geldanamycin. J Med Chem. 1999 Jan 28;42(2):260-6.[2]. McIlwrath AJ, Brunton VG, Brown R. Cell-cycle arrest and p53 accumulation induced by geldanamycin in human ovarian tumour cells. Cancer Chemother Pharmacol. 1996;37(5):423-8.[3]. Schnur RC, Corman ML, Gallaschun RJ, Cooper BA, Dee MF, Doty JL, Muzzi ML, Moyer JD, DiOrio CI, Barbacci EG, et al. Inhibition of the oncogene product p185erbB-2 in vitro and in vivo by geldanamycin and dihydrogeldanamycin derivatives. J Med Chem. 1995 Sep 15;38(19):3806-12.Geldanamycin Dilution CalculatorConcentration (start)xVolume (start)=Concentration (final)xVolume (final) femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter femtomolar picomolar nanomolar micromolar millimolar molar microliter milliliter liter C1V1C2V2calculateGeldanamycin Molarity CalculatorMass=ConcentrationxVolumexMW* picograms nanograms micrograms milligrams grams kilograms femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter g/molcalculate Chemical Properties Cas No. 30562-34-6SDF Download SDF Synonyms N/A Chemical Name [(3R,5S,6R,7S,8E,10S,11S,12Z,14E)-6-hydroxy-5,11,21-trimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate Canonical SMILES CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)C)OC)OC(=O)N)C)C)O)OC Formula C29H40N2O9 M.Wt 560.6 Solubility ≥16.9 mg/mL in DMSO, Storage Store at -20°CPhysical AppearanceA solidShipping ConditionEvaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon requestGeneral tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. BackgroundGeldanamycin, a crystalline antimicrobial compound derived from the culture filtrates of Streptomyces hygroscopicus var. geldanus var. nova., is a potent and specific inhibitor of heat shock protein 90 (Hsp90) that specifically binds to the unique ATP binding pocket of Hsp90 in a stable and pharmacologically specific manner. As an antimicrobial agent, geldanamycin exhibits moderate activity against protozoa, bacteria and fungi as well as parasite Syphacia oblevata and cell cultures of L-1210 and KB. Recent studies have shown that geldanamycin also inhibits the function of glucocorticoid receptor and endothelium-dependent relaxation of the rat aorta, mesentery and middle artery.ReferenceBucci M, Roviezzo F, Cicala C, Sessa WC, Cirino G. Geldanamycin, an inhibitor of heat shock protein 90 (Hsp90) mediated signal transduction has anti-inflammatory effects and interacts with glucocorticoid receptor in vivo. Br J Pharmacol. 2000; 131(1): 13-16.DeBoer C, Meulman PA, Wnuk RJ, Peterson DH. Geldanamycin, a new antibiotic. J Antibiot (Tokyo). 1970; 23(9):442-447.