.product-side h2 span {width: 50%;}.product-side h2 span.hover {}Related ProductsD-64131CHM 1PodophyllotoxinPX 12MPC 6827 hydrochlorideABT-751 (E7010)Molarity CalculatorDilution Calculator 2-Methoxyestradiol (2-MeOE2)Apoptotic, antiproliferative and antiangiogenic agentCatalog No.A4188SizePriceStockQty10mM (in 1mL DMSO)$50.00In stock 25mg$50.00In stock 50mg$110.00In stock 100mg$170.00In stock 800mg$800.00In stock Tel: +1-832-696-8203Email: sales@apexbt.comWorldwide Distributors Add to CartBulk InquiryFree samples Check OutSample solution is provided at 25 µL, 10mM.Product Citations1. Bao Y, Wang Z, et al. "A feed-forward loop between nuclear translocation of CXCR4 and HIF-1α promotes renal cell carcinoma metastasis." Oncogene. 2018 Sep 3.PMID:30177838Related Compound Libraries DiscoveryProbe™ FDA-approved Drug LibraryDiscoveryProbe™ Bioactive Compound LibraryDiscoveryProbe™ Anti-cancer Compound LibraryDiscoveryProbe™ Epigenetics Compound LibraryDiscoveryProbe™ Autophagy Compound LibraryDiscoveryProbe™ Angiogenesis LibraryQuality Control Quality Control & MSDS View current batch: 21 Purity = 98.25% COA (Certificate Of Analysis) HPLC NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Purity = 99.00% COA (Certificate Of Analysis) HPLC(Retest) NMR (Nuclear Magnetic Resonance) MSDS (Material Safety Data Sheet) Datasheet Chemical structure Related Biological Data Biological ActivityDescription2-Methoxyestradiol is a natural metabolite of estradiol.TargetsangiogenesisIC50ProtocolKinase experiment [1]:Microtubule depolymerizing activity assayThe effects of 2-Methoxyestradiol on cellular microtubule depolymerization were determined by indirect immunofluorescence techniques in rat aortic smooth muscle A-10 cells. Microtubules were visualized using a β-tubulin antibody. Three viewers determined the percent microtubule loss for each treatment concentration. The data were averaged and plotted as percent microtubule loss versus drug concentration and the EC50s for microtubule depolymerization were calculated from the log dose-response curves.Cell experiment [1]:Cell linesMDA-MB-435 and SK-OV-3 cellsPreparation methodThe solubility of this compound in DMSO is > 15.3 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.Reacting condition0 ~ 20 μM; 48 hrsApplicationsIn breast carcinoma MDA-MB-435 cells and ovarian carcinoma SK-OV-3 cells, 2-Methoxyestradiol inhibited cellular proliferation, with IC50 values of 1.38 μM and 1.79 μM, respectively.Animal experiment [2]:Animal modelsRats bearing 9L-V6R cellsDosage form60, 200 or 600 mg/kg/d; i.p.; for 9 daysApplicationsIn rats bearing 9L-V6R cells, 2-Methoxyestradiol significantly decreased HIF-1 activity and inhibited tumor growth in a dose-dependent manner (4-fold reduction for 60 mg/kg/day and 23-fold reduction for 600 mg/kg/day, respectively). The immunohistochemical staining results of tumor tissues further confirmed that 2-Methoxyestradiol dose-dependently down-regulated the gross HIF-1α protein levels. However, at the dose of 600 mg/kg/day, some drug related toxicity occurred, such as diarrhea and weight loss (12 ~ 15%).Other notesPlease test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.References:[1]. Rao PN, Cessac JW, Tinley TL, Mooberry SL. Synthesis and antimitotic activity of novel 2-methoxyestradiol analogs. Steroids. 2002 Dec;67(13-14):1079-89.[2]. Kang SH, Cho HT, Devi S, Zhang Z, Escuin D, Liang Z, Mao H, Brat DJ, Olson JJ, Simons JW, Lavallee TM, Giannakakou P, Van Meir EG, Shim H. Antitumor effect of 2-methoxyestradiol in a rat orthotopic brain tumor model. Cancer Res. 2006 Dec 15;66(24):11991-7.2-Methoxyestradiol (2-MeOE2) Dilution CalculatorConcentration (start)xVolume (start)=Concentration (final)xVolume (final) femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter femtomolar picomolar nanomolar micromolar millimolar molar microliter milliliter liter C1V1C2V2calculate2-Methoxyestradiol (2-MeOE2) Molarity CalculatorMass=ConcentrationxVolumexMW* picograms nanograms micrograms milligrams grams kilograms femtomolar picomolar nanomolar micromolar millimolar molar nanoliter microliter milliliter liter g/molcalculate Chemical Properties Cas No. 362-07-2SDF Download SDF Synonyms 2-methoxy Estradiol Chemical Name (8R,9S,13S,14S,17S)-2-methoxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol Canonical SMILES CC12CCC3C(C1CCC2O)CCC4=CC(=C(C=C34)OC)O Formula C19H26O3 M.Wt 302.41 Solubility ≥15.25 mg/mL in DMSO, ≥24.25 mg/mL in EtOH with ultrasonic, Storage Store at -20°CPhysical AppearanceA solidShipping ConditionEvaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon requestGeneral tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. Background2-Methoxyestradiol (2-MeOE2), an endogenous metabolite of 17β-estradiol (E2), is an inhibitor of microtubule assembly that inhibits the polymerization of tubulin and interferes with mitotic spindle dynamics leading to the blockage of mitosis of human cancer cells which lack estrogen receptors in metaphase. 2-MeOE2 is also an inhibitor of tumor growth and angiogenesis. Study results have shown that 2-MeOE2 induces mammalian cell transformation and genotoxicity in Syrian hamster embryo (SHE) fibroblasts through concentration-dependent inhibition of cell growth. Moreover, 2-MeOE2 has demonstrated anti-proliferative activity against estrogen-responsive breast cancer cell line MCF-7 and subsequent inhibition of the growth of tumors subcutaneously inoculated in mice.ReferenceTakeki Tsutsui, Yukiko Tamura, Makoto Hagiwara, Takashi Miyachi, Hirohito Hikiba, Chikahiro Kubo and J. Carl Barret. Induction of mammalian cell transformation and genotoxicity by 2-methoxyestradiol, an endogenous metabolite of estrogen. Carcinogensis 2000; 21(4): 735-740Hesham Attalla, Tomi P. Makela, Herman Adlercreutz and Leif C. Anderson. 2-Methoxyestradiol arrests cells in mitosis without depolymerizing tubulin. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1996; 228: 467-473