mRNA synthesis

In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)

TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide

Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)

A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain

Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails

Protect the integrity of proteins from multiple proteases and phosphatases for different applications.

Background文献引用Chemical Properties生物活性质量控制Background

FLAG标签肽（FLAG tag Peptide）是一个含有肠激酶酶切位点的8肽（Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys），专门用于免疫亲和层析。FLAG标签肽融合系统主要用于蛋白鉴定与纯化。融合蛋白的洗脱主要通过两种方式：以钙离子依赖的方式，通过抗体介导的亲和层析（降低pH）；用合成的多肽进行竞争性洗脱。FLAG标签肽允许在非变性的条件下进行洗脱。

标签肽必须有以下特点：第一，标签部分不应该干扰与其连接的蛋白质的天然折叠构象。第二，标签肽序列应该是水溶性的，应该最大程度的暴露于蛋白表面，以便其容易与配体相互作用。它应该适于温和且便宜的亲和纯化过程。最后，标签肽必须容易去除，从而获得天然产物。由于标签肽的小尺寸，可以由单个合成的寡核苷酸进行编码。在抗原抗体相互作用中，芳香族氨基酸起到重要的作用。

标签肽序列中第三位置的赖氨酸引导一个六肽序列，它确保该标签序列的最大亲水性。该亲水序列显示了强抗原性，也采用了高暴露的蛋白三维构象。FLAG的另一个优点是可以用肠激酶处理去除这个标签，得到含有真正N末端的融合蛋白。FLAG标签可以与融合蛋白的N端或C端进行融合。然而，N端融合有几个优点。抑制ELISA实验表明，当第一个氨基酸的α-氨基游离时，抗-Flag抗体M1可以结合3-4个Flag。

参考文献：1. A. Einhauer, A. Jungbauer. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49 2001 455 465. 2. Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A 1981;78:3824 8.3. Hopp TP. Protein surface analysis: methods for identifying antigenic determinants and other interaction sites. J Immunol Methods 1986;88:1 18.4. Power BE, Ivancic N, Harley VR, Webster RG, Kortt AA, Irving RA, et al. High-level temperature-induced synthesis of an a

文献引用1. Ter Beek J, Parkash V, et al. \"Structural evidence for an essential Fe-S cluster in the catalytic core domain of DNA polymerase ϵ.\" Nucleic Acids Res. 2019 Jun 20;47(11):5712-5722.PMID:309681382. Wang M, Hu J, et al. \"High glucose-induced ubiquitination of G6PD leads to the injury of podocytes.\" FASEB J. 2019 Feb 20:fj201801921R.PMID:307858023. Zi-Wei Chen, John R Bracamontes, et al. \"Multiple Functional Neurosteroid Binding Sites on GABAA Receptors.\" bioRxiv. 2018 June 29.4. haoyun Zhang, Meng Wang, et al. \"High glucose-induced ubiquitylation of G6PD leads to the injury of podocyte.\" bioRxiv. 2018 June 20.5. Heredia JD, Park J, et al. \"Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning.\" J Immunol. 2018 Apr 20. pii: ji1800343.PMID:296789506. Sarah Zernia, Ronny Frank, et al. \"Surface‐Binding Peptide Facilitates Electricity‐Driven NADPH‐Free Cytochrome P450 Catalysis.\" ChemCatChem. 2017 November19.7. Banks CJ, Rodriguez NW, et al. \"Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-mediated Inhibition of Mitochondrial Respiration.\" Mol Cell Biol. 2017 Jul 24. pii: MCB.00354-17.PMID:287398578. Lopez J, Bessou M, et al. \"Mito-priming as a method to engineer Bcl-2 addiction.\" Nat Commun. 2016 Feb 2;7:10538.PMID:26833356Hide ↑↑Chemical PropertiesPhysical AppearanceA solidStorageDesiccate at -20°CM.Wt1012.97Cas No.98849-88-8FormulaC41H60N10O20SynonymsH-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OHSolubility≥50.6 mg/mL in DMSO, ≥210.6 mg/mL in H2O, ≥34.03 mg/mL in EtOHChemical NameFLAG PeptideSDFDownload SDFCanonical SMILESC1=CC(=CC=C1CC(C(=O)NC(CCCCN)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCCCN)C(=O)O)NC(=O)C(CC(=O)O)N)O运输条件试用装：蓝冰运输。 其他可选规格：常温运输或根据您的要求用蓝冰运输。一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。生物活性FLAG标签肽是一个含有肠激酶酶切位点的8肽，可用于重组蛋白的纯化。

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