GelShift,orBandShiftAssay,orElectrophoreticMobilityShiftAssay(EMSA)isatechniqueforstudyinggeneregulationanddeterminingprotein:DNAinteractions.TheassayisbasedontheobservationthatcomplexesofproteinandDNAmigratethroughanon-denaturingpolyacrylamidegelmoreslowlythanfreeDNAfragmentsordouble-strandedoligonucleotides.Thegelshiftassayiscarriedoutbyfirstincubatingaprotein(s)(suchasnuclearorcellextract)witha32Pend-labeledDNAfragmentcontainingtheputativeproteinbindingsite.Thereactionproductsarethenanalyzedonanondenaturingpolyacrylamidegel.ThespecificityoftheDNA-bindingproteinfortheputativebindingsiteisestablishedbycompetitionexperimentsusingDNAfragmentsoroligonucleotidescontainingabindingsitefortheproteinofinterestorotherunrelatedDNAsequences.
行业新闻
- 进口Binding Site品牌清关代理 进口immunologicals现货
- Binding Site_来宝网
- bindingsite|产品目录|代理商|上海玉博生物科技有限公司
- binding site是什么意思_binding site怎么读_binding site翻译_用法...
- 【binding_site】什么意思_英语binding_site的翻译_音..._有道词典
- binding site是什么意思_binding site在线翻译_英语_读音_用法_例句...
- Binding Site Binding Site厂家
- Binding Site产品促销 immunologicals授权代理商，正品
- binding site是什么意思_binding site怎么翻译及发音_用法
- The Binding Site与Affymetrix eBioscience签订临床流式细胞仪试剂合 ...
- Binding Site公司 Binding Site厂家
- Binding Site型号参数规格 immunologicals代理
Band Shift Assay
