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产品说明
DescriptionMicroarray PanelLung carcinoma tissue microarray (slide 5 of 1040 cases of lung cancer survey slide set), containing 102 cases of squamous cell carcinoma, 17 adenosquamous carcinoma, 76 adenocarcinoma, 9 small cell carcinoma, 4 bronchioloalveolar carcinoma, single core per caseCores208LC20816 H&ECases208Row number13 Column number16Core Diameter (mm)1Thickness (µm)5QA/QCAnti-Actin confirmedTissue Array TypeFFPESpeciesHumanApplicationsRoutine histology procedures including Immunohistochemistry (IHC) and In Situ Hybridization (ISH), protocols which can be found at our support page.Notes1. TMA slides were sectioned and stored at 4°C and may not be fresh cut, but still suitable for IHC. Please request fresh cut if experiment involves phospho-specific antibodies, RNA studies, FISH or ISH, etc. A minimum of 3 slides per TMA must be purchased to cover the cost of trimming for fresh sectioning.2. Most TMA slides were not coated with an extra layer of paraffin (tissue cores can be easily seen on the glass). To prevent tissue detachment during antigen retrieval, unbaked slides must be baked for at least 30 to 120 minutes at 60°C. before putting into xylene for de-paraffinization. Baked slides were sent out baked for 2 hours.In the following specsheet,“*” means invalid core; “-” means no applicable or negative in IHC markers.Mouseover and click individual cores to view high resolution images.12345678910111213141516ABCDEFGHIJKLM
biomax的IHC和ISH
免疫组织化学(IHC)和原位杂交(ISH)服务为组织阵列实验有特定需求的客户提供。通过使用客户提供的抗体,DNA片段或寡核苷酸在正常和患病的人体组织上进行定位,从而定位感兴趣的蛋白质/基因,这些组织选自US Biomax广泛选择的组织阵列和组织库。这些服务具有很高的成本效益,并按最高标准执行,可帮助您了解基因/蛋白质的功能或鉴定和验证潜在的诊断和治疗靶标。结果以可发布的详细病理报告的形式显示,并附带高分辨率的数码照片。
US Biomax使用自动IHC染色机(Ventana)获得高染色质量,从而确保可重复的结果。该研究平台允许定制试剂和方案以获得最佳结果。我们提供多种检测扩增水平,允许进行高灵敏度的测定,以确保没有假阴性。与基于生物素的检测系统相比,链霉亲和素-辣根过氧化物酶(HRP)的使用可最大程度地减少非特异性结合。
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