The Exonuclease-It plasmid purification system isdesigned to selectively digest linear chromosomal DNA contaminationin a plasmid preparation, without significantly degradingany supercoiled plasmid (or any covalently closed circularplasmid). Exonuclease-It will also degrade open circularplasmid and circular single stranded DNA. The Exonuclease-Itenzyme reagent contains a proprietary mixture of exonucleasesto accomplish this task. In a typical 6 hour incubation,Exonuclease-It will reduce the amount of chromosomalDNA contamination in a plasmid preparation by 98 percent.The by-products of the exonuclease digestion are deoxynucleosidemonophosphates. The Exonuclease-It plasmid purificationsystem is ideally used as an added step in any plasmid purificationmethod, in order to thoroughly remove chromosomal DNA. Thiskit contains sufficient reagents for purifying 150 mg ofplasmid. Exonuclease-It is not for use in purifyingplasmid for human therapy. The primary application is for removal of chromosomal DNAcontamination from plasmid preparations. However, Exonuclease-Itmay be used in any application to selectively digest linearDNA without significantly degrading covalently closed circulardouble stranded DNA. For example, a mixture of linear DNAand covalently closed circular DNA may be generated by aligation reaction. Exonuclease-It can selectivelydigest the linear DNA vector prior to transforming cells,in order to reduce background transformants. Exonuclease-Itmay be used after Supercoil-It . The Supercoil-Itincubation converts open circular plasmid to supercoiledform, and the subsequent Exonuclease-It incubationremoves chromosomal DNA contamination. Supplied: Exonuclease-Itis supplied with sufficient 40X buffer. Low Price:Purifies 150 mg of plasmid for only $200. Shipping: dryice.

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