Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino group to create a secondary amine. As a consequence of its cyclic structure, proline constrains the structure of proteins where it occurs, disrupting alpha-helices. Isomerization between the cis and trans forms of proline in proteins is isomerized by peptidyl-prolyl isome......阅读全文 抗CyPA自身抗体与SLE关系的探 作者：陈英剑 李芳秋摘要 以ｒｈＣｙＰＡ为包被抗原，用间接ＥＬＩＳＡ检测了４５例ＳＬＥ病人血浆抗ＣｙＰＡ自身抗体的ＩｇＧ、ＩｇＡ、ＩｇＭ三种类型，并探讨了此抗体与多项临床指标的相关性。结果显示，ＩｇＧ、ＩｇＡ、ＩｇＭ三类抗ＣｙＰＡ自身抗体的阳性率分别为３６％，２０％，１８％，总阳性率为４８％。存在皮 Adhesion and Diapedesis of Lymphocytes Cell adhesion is a fundamental feature of multicellular organisms including their defense mechanisms. In the later case in mammals, leukocytes play ce 通过反硝化细菌和激光同位素分析仪测定溶解硝酸盐中... 通过反硝化细菌和激光同位素分析仪测定溶解硝酸盐中的δ15N和δ18OLGR氧化亚氮同位素分析仪测量水中硝酸盐成功案例：Analytical Chemistry 文章：Combining Denitrifying Bacteria and Laser Spectroscopy for Isotopic biosynthesis of Chorismate in Bacteria and Plants The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio Chromosomal DNA Extraction from Gram-positive Bacteria Chromosomal DNA Extraction from Gram-positive BacteriaThis procedure was originally developed forListeria monocytogenesbut has worked well General Reference Units1 mg = 10-3 g1 ug = 10-6 g1 ng = 10-9 g1 pg = 10-12 g1 kb of double stranded DNA = 660 kD1 kb of single stranded DNA = 330 kD1 kb of Maintenance of Probes in bacteria including Escherichia coli Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on Monocyte and its Surface Molecules Monocytes are a class of phagocytes involved in non-specific immune defense that develop into macrophages. In plasma, monocytes ingest and destroy bac Plasmid Miniprep MaterialsSolution II: 0.2N NaOH/1% SDSSolution III: 3 M KOAc, pH4.8RNAseA (DNAse free) 10 µg/mLChloroform/Isoamyl alcohol (1/25 v/v)Isopropanol70 % et 欧盟评估一种葡萄糖氧化酶的安全性据欧盟食品安全局（EFSA）消息，近日，欧盟食品安全局就一种葡萄糖氧化酶（glucose oxidase）用于烘焙过程的安全性发布意见。 这种葡萄糖氧化酶是利用转基因米曲霉产生的食品酶。欧盟食品安全局专家组对这种葡萄糖氧化酶的安全性进行了评估。专家组经过评估认为，这种葡萄糖氧化酶在预期最大使 Steps in the Glycosylation of Mammalian N-linked Oligosaccarides The biosynthesis pathway of N-glycans is a costly system with respect to the number of enzymes that are involved in the synthesis and trimming of N-gl Science杂志最受关注的文章（1月）美国的《Science》杂志由爱迪生投资创办，是国际上著名的自然科学综合类学术期刊，与英国的《Nature》杂志被誉为世界上两大自然科学顶级杂志。Science杂志主要发表原始性科学成果、新闻和评论，许多世界上重要的科学报道都是首先出现在Science杂志上的，比如艾滋病与人类免疫缺陷病毒之间的 如何使用植物表型成像系统完成十字花科表型研究的论文 最近，来自比利时根特大学的专家利用WIWAM XY植物表型系统发表了题为Drought resistance is mediated by divergent strategies in closely related Brassicaceae的文章，发表在植物学著名期刊New Phytol Catabolic pathways for alanine, glycine, serine, cysteine, tryptophan Amino acids in the diet have one of two fates - either they are incorporated into proteins or they are broken down for energy and metabolic intermedia 上海交通大学连续在Science/Cell/NEJM等发表11项成果2019软科世界大学学术排名于2019年8月15日18时正式发布。排名展示了全球领先的1000所研究型大学，中国内地共有132所大学上榜，上海交通大学首次跻身世界百强，位列世界第82。另外，在8月16日，国家自然科学基金委员会公布了基金情况，上海交通大学所获得资助项目及金额都是位居全国前列。 2019年 上海交大不止发了8篇CNS 还拿了国自科大额资助2019软科世界大学学术排名于2019年8月15日18时正式发布。排名展示了全球领先的1000所研究型大学，中国内地共有132所大学上榜，上海交通大学首次跻身世界百强，位列世界第82。另外，在8月16日，国家自然科学基金委员会公布了基金情况，上海交通大学所获得资助项目及金额都是位居全国前列。 李灿院士 江雷院士 黄维院士 郎建平院士等成果速递1. Nature Chem.：双重电催化可实现共轭烯烃的对映选择性氢氰化 手性腈及其衍生物广泛存在于药物和生物活性化合物中。对映选择性烯烃氢氰化反应是合成这些分子的一种方便有效的方法。然而，目前仍然在研究以宽底物范围和高官能团耐受性为特征的普遍适用的方法。近日，康奈尔大学Robert A. Experimental Surgery-3 Antibiotic GuidelinesGeneral RecommendationsSelectionIf a culture and sensitivity is not available, select antibiotics based on probable organism and 赵家军教授团队在胆固醇代谢研究领域取得重要进展卵泡刺激激素（FSH）是腺垂体分泌的一种糖蛋白激素，其主要作用是促进卵巢合成与分泌雌激素【1】。在女性绝经过程中，体内FSH的水平随着卵巢功能衰竭而升高【1】。绝经对女性的健康及生活质量具有严重的影响，导致女性非酒精性脂肪肝、血脂紊乱、心血管疾病及骨质疏松等疾病发病率显著升高【3-5】。近年来的 玉米Proline responding 1(pro1)突变在蛋白合成和细胞...（一） 玉米Proline responding 1(pro1)突变在蛋白合成和细胞周期调控中起到关键作用上海大学生命科学学院、上海市能源作物育种及应用重点实验室的研究人员证实，玉米Pro1基因(Zm P5CS2)的突变造成了突变体细胞中脯氨酸(proline)合成受阻，从而导致proline积累的减少。突 Auxin Immunolocalization in Plant Tissues The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expressio Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM(Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 细胞组分和细胞器——细胞器分离 Labeling Microtubules(Molecular Dynamics Inc.)Microtubules are involved in many aspects of cell motion including propulsion, mitosis E-Z 96® M13 Isolation Vacuum Manifold Protocol 实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids fr 做这5件事情能让机体的微生物组更加健康！新年伊始，人们关注自身的健康状况是最平常不过的事情了，但很少有人会认为，生活在机体肠道中的微生物组会对机体健康至关重要。肠道中的这些细菌到底有多重要呢？我们体内的细菌细胞的数量与机体细胞一样多，其能帮助控制很多事件，比如炎症、癌症的发生和疗法、以及我们如何从食物中获取能量、甚至我们对食物的渴望和 Polyadenylation of mRNA Gene expression requires the coordination and integration of multiple processes, including transcription, splicing, polyadenylation, nucleocytoplasmic Manipulating Expression of Tonoplast Transporters Plant vacuoles have multifaceted roles including turgor maintenance, cytosolic pH and ionic homeostasis, plant protection against environmental st IL-10 Anti-inflammatory Signaling Pathway IL-10 is a cytokine with potent anti-inflammatory properties, repressing the expression of inflammatory cytokines such as TNF-alpha, IL-6 and IL-1 by 2019年搞笑诺贝尔奖：吃意大利披萨竟能降低死亡率？北京时间9月13日，第29届搞笑诺贝尔奖颁奖典礼在美国哈佛大学桑德斯剧院举行，今年的搞笑诺贝尔奖又有哪些让你意想不到的奖项呢？让我们一睹为快！ 医学奖 获奖者：Silvano Gallus 获奖研究：研究表明，吃披萨或能够帮助预防疾病并降低死亡风险，但前提是要在意大利境内食用在意大利制作的 Hemoglobin\'s Chaperone The function of hemoglobin in oxygen transport by red blood cells requires precise assembly of a tetramer of two beta-subunits and two alpha-units. If