Antibodies bind specifically to the antigens that induce their production. This specificity is an advantage when identifying and quantifying proteins in an unknown sample mixture. This is the basis for immunoassays such Western blots and enzyme linked immunosorbent assays (ELISA). However, in some cases structural similarities between proteins and interference from diluent ingredients can lead to nonspecific binding resulting in high background and false positive signals. This is very common problem with polyclonal antibodies, and in  multiplexed assays.Biotin and streptavidin offer solution for improving specificity and reducing background without disrupting the normal biological function of the proteins.   Biotin (Vitamin B7) is a water-soluble vitamin which covalently binds to and stabilizes proteins in a biological system. Due to its small molecular weight and simple chemical structure  biotin conjugation does not affect the biological function of bound proteins. Power and versatility make flow cytometry a cornerstone in modern vaccinology and immunology. One of the most powerful application of this platform is for evaluate drug and vaccine efficacy. Multiparameter flow cytometry relies on the use of fluid systems, fluorescent proteins, and optical systems to detect and collect fluorophore signals. IBT CEO Dr. M. Javad Aman will discuss the development of a multi-component toxoid vaccine for S. aureus and the regulatory challenges and opportunities when moving a product from pre-clinical to clinical stage. The live webinar scheduled for Sept. 24, 2020 will feature experts on various aspects of antimicrobial resistance To address the urgent and immediate need for R D tools for the fight against the public health threat from SARS-CoV-2, IBT Bioservices now offers a pseudotype virus system to assay inhibition of infectivity in a BSL-2 environment. Briefly, Vesicular Stomatitis virus (VSV) glycoprotein gene (G) has been substituted with SARS-CoV-2 Spike Protein (rVSV pseudotyped SARS-CoV-2 Spike). The recombinant rVSV-DG SARS-CoV-2 Spike has been incorporated into a convenient luciferase-based neutralization assay for evaluating the efficacy of drug candidates that target Spike-mediated infection (Figure 1). This system is similar to previously published and validated VSV pseudotype platform for Ebolavirus and Marburgvirus1,2,3. The increasing rate of bacterial resistance creates a challenging environment for the development of therapies for bacterial infections. Bacterial skin infections are one of the leading manifestations of infectious disease in the world. Staphylococcus aureus—both Methicillin-resistant (MRSA) and Methicillin-Sensitive (MSSA) strains—is the leading cause of skin and soft tissue infections in the USA. It is the leading cause of hospital associated (HA) and community associated (CA) infections worldwide. In recent years, animal-to-human crossovers have been observed with Nipha virus in Malaysia, Ebola and Marburg viruses in Africa. SARS-CoV-2 is just one among three 21st century animal-to-human Coronavirus spillover events. Considering the high rate of mutation among RNA viruses, the number of animal coronaviruses, and the mixing of animals into densely populated areas spillover is not unexpected. Flow cytometry uses light scattering, light excitation and emission of fluorochrome molecules to gather detailed information about specific cells or particles of interest. Using antibody-fluorophore conjugates flow cytometry allows researchers to draw phenotype-dependent conclusions about cellular phenotype and function.  The fluorophores present on bound antibodies excited by lasers yield detailed IBT Vaccines, a wholly owned subsidiary of Integrated Biotherapeutics Inc., announces it has received $3.9 million to advance the development of the IBT-V02 vaccine for Staphylococcus aureus induced recurrent skin and skin structure infections (SSSI). The funding is part of a $48 mil. investment in 8 companies by Novo Holding\'s REPAIR According to WHO statistics, seasonal influenza viruses cause 3-5 million cases of infection with 290,000-650,000 deaths worldwide each year. There are 4 types of seasonal influenza viruses. Types A (which can infect and be transmitted between birds and some mammals such as pigs and humans) and B (which only infects 4 November 2019INTEGRATED BIOTHERAPEUTICS TO PRESENT ON NOVEL VACCINES FOR MRSA AT WORLD ANTI-MICROBIAL CONGRESS 2019Integrated Biotherapeutics, Inc. @ World Anti-Microbial Resistance Congress 2019   AN INDUSTRY EXPERTS CONFERENCE ON COMBATING ANTI-MICROBIAL RESISTANCE Maryland — 4 November 2019 – Integrated Biotherapeutics CEO Dr. M. Javad Aman will Subscribe to the IBT Bioservices Tech Talk list and receive periodic updates on industry news and IBT Bioservices products and services. ADDRESS: 4 Research Ct, Suite 300 Rockville, MD 20850(877) 411-2041 Survival and weight change in BALB/c mice challenged with INFV A/ Texas/36/91 (H1N1) and treated with antiviral Osletamivir Phosphate (Tamiflu) Lung viral load and Survival (30 % weight loss cut-off) in BALB/c mice challenged with INFV H3N2 A/HK/1/68. Alpha (UK) B. 1.1.7 / 501Y.V1amino acid mutations: del69–70 HV, del144 Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H Beta (South Africa) B.1.351amino acid mutations: K417N, E484K, N501Y, D614G, A701V Gamma (Brazil) P.1amino acid mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I