ExoBrite™ EV 膜染色剂和抗体能否同时对外泌体进行染色？常见问题解答Medium & CoverGrip™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色其他 DNA /RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack®背景还原剂酪胺信号放大（CF® 染料和其他酪胺）提出问题是的，可以使用 ExoBrite™ EV 膜染色剂和荧光抗体同时对外泌体和 EV 进行染色。对于纯化的外泌体，当外泌体在 1 中染色时，我们已经看到了良好的结果mL 的 1X ExoBrite™ 加 1 ug 荧光抗体。对于珠子结合的外泌体，我们发现先用一抗染色，然后用 ExoBrite™ EV 膜染色，中间有一个洗涤步骤，可以得到最好的结果。当染色同时进行时，抗体信号不受影响，但 ExoBrite™ 信号减少约 25%。当珠子结合的外泌体在 100 uL 10X ExoBrite™ 加 1 ug 荧光抗体中染色时观察到这一点；使用不同比例的 ExoBrite™ 和抗体可能会产生不同的结果。查看更多常见问题解答使用 EverBrite™ 封固剂时，我看到了高紫外线/蓝色背景的自发荧光。这是预期的吗？如何减少？常见问题解答类别细胞凋亡、坏死和细胞活力试剂盒CellBrite® & MemBrite® 膜和细胞表面染色剂CF® 染料DNA 和 RNA 定量试剂盒DNAzure® 可见 DNA 凝胶染色剂 EvaGreen® 染料和预混液EverBrite™ 封片剂和 CoverGrip™ 盖玻片密封剂外泌体和 EV染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶检测微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他 DNA/RNA 凝胶染色剂 PMA 和 PMA xx™ 为viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ 和 DNAstorm™ FFPE 提取试剂盒二抗 TrueBlack® 背景降低剂酪胺信号放大（CF® 染料和其他酪胺）提出问题EverBrite™ 封固剂在紫光激发下确实显示出高背景荧光。当使用 UV 激发和 DAPI 滤光片立方体使用落射荧光成像时，背景更加突出，但在使用 405 nm 激光的共聚焦成像中背景通常很低。查看更多常见问题解答CoverGrip™ 盖玻片密封剂是否可去除？常见问题解答EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶检测微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他 DNA/RNA 凝胶染色剂 PMA 和 P MAXX™用于活力 PCR 产品运输、储存、保质期和溶解度蛋白质检测和分析刃天青细胞活力测定 RNAstorm™ 和 DNAstorm™ FFPE 提取试剂盒二抗 TrueBlack® 背景降低剂酪胺信号放大（CF® 染料和其他酪胺）提出问题CoverGrip™ 旨在替代指甲油以永久密封湿封盖玻片。它可以通过将载玻片浸泡在缓冲液中来去除（有关详细信息，请参阅产品信息表）。 CoverGrip™ 并非设计用于为杂交创建可移动的临时密封件。查看更多常见问题解答封片后是否可以去除样品中的 EverBrite™ 封片剂？常见问题解答& CoverGrip™ 盖玻片密封胶外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架站insOther DNA/ RNA Gel StainsPMA 和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducerTyramide Signal Amplification (CF® Dye & Other Tyramides)Ask a questionEverBrite™ 湿式封片剂是一种水性、含甘油的介质。它可以通过用 PBS 或其他缓冲液清洗几次从样品中去除。但是，用 PBS 清洗不会去除 DAPI 染色。对于使用 EverBrite™ Hardset 或 EverBrite™ 湿固培养基固定在载玻片上的盖玻片，可以将载玻片浸泡在载玻片罐中的 ​​PBS 中数小时，同时轻轻搅拌，直到盖玻片滑落（避免撬动或拉动盖玻片，这可能会损坏样品）。然后多次清洗载玻片以去除任何残留的封固剂。如果盖玻片已用 CoverGrip™ 盖玻片密封剂密封，浸泡在 PBS 中也会去除密封剂。可能需要其他方法来去除其他类型的盖玻片密封剂。查看更多常见问题解答Viafluor™  SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and can be used to monitor cell division by flow cytometry.

Viafluor™  SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and can be used to monitor cell division by flow cytometry.

The membrane permeable compound is non-fluorescent until it enters viable cells, where it is hydrolyzed by cytoplasmic esterase enzymes to releases the fluorescent amine-reactive dye. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell, while excess unreacted dye is washed away.  Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labeling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analyzed immediately after staining (Figs. 1 and 2).  Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining also can withstand fixation and permeabilization for subsequent immunostaining.

Kit Components:

Each kit contains 10 lyophilized dye vials, anhydrous DMSO for preparing stock solutions, and a labeling protocol. One kit can be used to label at least 1.8×10⁸ cells; the final number of assays that can be performed per kit depends on the dye concentration used (see product protocol under downloads).

ViaFluor kits come in 3 dye options:

• ViaFluor™ CFSE (also known as CFDA-SE) can be detected in the FITC channel.
• ViaFluor™ 405-SE can be detected in the Pacific Blue channel.
• ViaFluor™ 568-SE can be detected in the Texas Red or PE-Texas Red channels.

ViaFluor™ CFSE can be used as a cytoplasmic stain in yeast. However ViaFluor™ 405-SE and ViaFluor™ 568-SE do not stain well in yeast.

Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE or CFSE) is also available separately.

References

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