为什么我会看到微弱的荧光、随着时间的推移染料性能下降，或者染色后凝胶上残留一层染料？常见问题解答KitsDNAzure® Visible DNA Gel StainEvaGreen® Dye and Master MixesEverBrite™ Mounting Medium & CoverGrip™ Coverslip SealantExosome & EV StainingGelRed® and GelGreen® Nucleic Acid Gel StainsGelRed® and GelGreen® TroubleshootingIon Indicators & ChelatorsLuciferase AssaysMicrobiology: Dyes for bacteria and yeastMix-n- 试剂盒染色™抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他 DNA/RNA 凝胶染色剂用于活性 PCR 的 PMA 和 PMAxx™ 产品运输、储存、保质期和溶解度蛋白质检测与分析刃天青细胞活性测定RNAstorm™ 和 DNAstorm™ FFPE 提取试剂盒二抗TrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)Ask a question有几种可能性：染料可能已经从溶液中沉淀出来。将 GelRed® 或 GelGreen® 溶液加热至 45-50°C 两分钟，然后涡旋溶解。在室温下储存染料以避免沉淀。如果您看到凝胶的高背景染色，则您使用的琼脂糖可能质量低下。琼脂糖中的污染物可能会与染料结合，导致背景增加。查看更多常见问题解答如何使用 GelRed® 和 GelGreen®？常见问题解答盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他 DNA/ RNA 凝胶染料 PMA和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification(CF® Dye & Other Tyramides)提问 GelRed® 和 GelGreen® 可以在凝胶浇注过程中以 1X 的终浓度添加到琼脂糖中，或者它们可以以 3X 的终浓度在水中用于电泳后凝胶染色。如需详细的使用方案，请下载 GelRed® 产品信息表或 GelGreen® 产品信息表。查看更多常见问题解答为什么你们提供溶解在 DMSO 或水中的 GelRed® 和 GelGreen®？常见问题解答Medium & CoverGrip™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色其他 DNA /RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide信号放大（CF® 染料和其他酪胺）提出问题与 DMSO 中的库存相比，水配方是一种更新和改进的产品。我们建议在水中使用染料，以避免处理可通过皮肤吸收的 DMSO 的潜在危害。我们继续提供在 DMSO 中的染料，因为一些用户不希望改变他们既定的实验室协议。根据内部测试，两种配方的性能相似。查看更多常见问题解答Biotium 的哪些抗体可用于染色微珠结合的外泌体？常见问题解答类别细胞凋亡、坏死和细胞活力试剂盒CellBrite® 和 MemBrite® 膜和细胞表面染色剂CF® 染料 DNA 和 RNA 定量试剂盒DNAzure® 可见 DNA 凝胶染色剂 EvaGreen® 染料和预混液EverBrite™ 封片Medium & CoverGrip™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色其他 DNA /RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)Ask a question我们已经测试了我们所有的四跨膜蛋白（CD9、CD63 和 CD81）和 Ep-CAM 抗体克隆在流式细胞术中对与 CD63 捕获磁珠结合的外泌体进行染色的能力.效果良好的克隆列在\"外泌体和 EV 标记”技术页面的表格中。https://biotium.com/technology/exosome-ev-labeling/#antibodies 查看更多常见问题解答Biotium 的哪些抗体可用于染色纯化的外泌体？常见问题解答类别细胞凋亡、坏死和细胞活力试剂盒CellBrite® 和 MemBrite® 膜和细胞表面染色剂CF® 染料 DNA 和 RNA 定量试剂盒DNAzure® 可见 DNA 凝胶染色剂 EvaGreen® 染料和预混液EverBrite™ 封片剂和CoverGrip™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他DNA/RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide 信号放大（CF® 染料和其他酪胺）提出问题我们已经测试了我们所有的四跨膜蛋白（CD9、CD63 和 CD81）抗体克隆对通过流式细胞术中尺寸排阻色谱 (SEC) 纯化的外泌体进行染色的能力。效果良好的克隆列在\"外泌体和 EV 标记”技术页面的表格中。https://biotium.com/technology/exosome-ev-labeling/#antibodies 查看更多常见问题解答NucView™ 530 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

NucView™ 530 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView™ caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. NucView™ substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView™ Caspase-3 Substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The staining is also formaldehyde-fixable.

NucView™ 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence (Ex/Em 528/563 nm, Figure 1), for detection in the Cy®3 channel by fluorescence microscopy (Figure 2) or the PE channel by flow cytometry (Figure 3). NucView™ 530 can be used for multi-color imaging with blue, green, or far-red fluorescent probes. Note that when excited by the 488 nm laser line, NucView™ 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.

NucView™ 530 Caspase-3 Substrate is available as a 1 mM solution in DMSO (catalog no. 10406) or PBS (phosphate-buffered saline). The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView™ 530 staining.

Biotium also offers blue fluorogenic NucView™ 405 Caspase-3 Substrate, and green fluorogenic NucView™ 488 Caspase-3 Substrate and kits.

Figure 1. Excitation and emission spectra of NucView™ 530 dye with dsDNA.

Figure 2. Detection of mitochondrial membrane potential, caspase activity, and apoptosis in MCF-7 cells. Cells were either untreated (A) or treated with staurosporine overnight to induce apoptosis (B), then stained with MitoView™ Blue, NucView™ 530 Caspase-3 Substrate, and CF™488A Annexin V for 30 minutes at 37^oC in cell culture medium with no wash. Healthy cells show mitochondrial staining with MitoView Blue (cyan). Apoptotic cells lose MitoView™ Blue staining (cyan), and show NucView™ 520 staining of nuclei (red) and CF™488A Annexin V staining of cell membranes (green).

Figure 3. Jurkat cells were treated with DMSO (negative control, blue) or 0.1 uM, 0.25 uM, or 1 uM staurosporine (red) to induce apoptosis in the presence of NucView™ 530 and CF™405M Annexin V for four hours at 37^oC before analysis on a BD LSR II flow cytometer. Staining with NucView™ 530 and CF™405M Annexin V increase with increasing concentration of staurosporine.

Cy dye is a registered trademark of GE Healthcare.