技术提示：使用 EvaEZ™ 荧光聚合酶活性测定试剂盒获得成功的方案和技巧相关DEvaEZ™ 荧光聚合酶活性测定试剂盒PCR 和 DNA 扩增DNA 定量试剂盒、核酸定量和提取DNA/RNA 纯化试剂盒、DNA 凝胶染色剂和附件DNA/RNA 纯化试剂盒、核酸酸定量和提取RNA 定量试剂盒，核酸定量和提取qPCR 试剂DNA 定量试剂盒RNA 定量试剂盒DNA 凝胶染色及相关产品DNA 结合染料活性DNA/RNA 结合染料DNA/RNA 纯化试剂盒分享文章与同事分享这篇文章。复制链接发送电子邮件EvaEZ™ 是一种基于荧光的测定使您无需使用放射性同位素即可定量 DNA 聚合酶活性。虽然该测定易于使用，但有许多因素会影响您的数据质量。在本文中，我们概述了使用我们的 EvaEZ 获得最佳结果的建议™ 用您的酶进行测定。文章​​包括：设置仪器和样品的提示使用标准酶滴定进行测定计算初始斜率和绘制标准曲线计算核苷酸掺入在 EvaEZ™ 测定中使用热启动聚合酶优化测定线性度和提高准确性的提示注册到我们的电子通讯接收此技术提示的完整版本我应该增加厚组织切片的酪胺显影时间吗？常见问题解答 类别细胞凋亡、坏死和细胞活力试剂盒CellBrite® & MemBrite® 膜和细胞表面染色剂CF® 染料 DNA 和 RNA 定量试剂盒DNAzure® 可见 DNA 凝胶染色剂 EvaGreen® 染料和预混液EverBrite™ 封片剂& CoverGrip™ 盖玻片密封胶外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架站insOther DNA/ RNA Gel StainsPMA 和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducerTyramide 信号放大（CF® 染料和其他酪胺）提出问题我们没有关于增加酪胺孵育时间是否会改善较厚切片染色的任何第一手信息。我们已经在 10 um 厚的载玻片冷冻切片上测试了酪胺。已发布的 40 um 浮动切片方案建议在室温下执行酪胺显影步骤 15 分钟。如果您使用自由浮动切片，通常所有孵育步骤（透化、抗体孵育和洗涤）都比载玻片上的切片长。酪胺是小分子，应该比抗体偶联物更快地穿透组织，但您可能希望测试更长的孵育时间以使缓冲液穿透组织。查看更多常见问题解答我可以孵育多种一抗并结合酪胺扩增吗？常见问题解答™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他DNA/RNA 凝胶StainsPMA 和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducerTyramide Signal Amplification (CF® Dye & Other Tyramides)Ask a question我们的酪胺放大试剂盒已被证明对多色荧光成像具有稳健性和多功能性，与染料标记的抗体和各种细胞染色方法兼容（见图 1）。除了一种或多种染料标记的抗体外，还使用酪胺扩增试剂盒，按照试剂盒协议固定和封闭样品；用一抗标记；然后使用二抗检测一抗。染料标记的二抗可以与酪胺试剂盒中的 HRP 偶联二抗或 HRP-链霉亲和素共同孵育。洗涤后，根据试剂盒方案进行 CF® Dye 酪胺反应。酪胺反应不会干扰染料标记的抗体或其他荧光染色试剂的结合。在同一样品上使用一种以上染料酪胺进行多色检测需要连续的酪胺染色反应，遵循在每个步骤之间通过 HRP 灭活或抗体剥离进行编辑。请参阅我们的技术提示：使用 Biotium 的酪胺扩增试剂盒进行多色荧光成像查看更多常见问题解答From: 106Sizes: 5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug labeling, 50-100 ug labelingCatalog #: 92433, 92432, 92431, 92430, 92429, 92428, 92427, 92426, 92425, 92230, 92231, 92232, 92233, 92234, 92235, 92236, 92237, 92238, 92239, 92240, 92241, 92242, 92243, 92245, 92246, 92247, 92248, 92250, 92251, 92252, 92253, 92254, 92255, 92256, 92257, 92258, 92259, 92260, 92261, 92262, 92263, 92264, 92265, 92267, 92268, 92270, 92271, 92272, 92273, 92274, 92275, 92276, 92277, 92278, 92279, 92280, 92281, 92282, 92283, 92284, 92285, 92287, 92288, 92289, 92290, 92291, 92303, 92304, 92305, 92316, 92317, 92318, 92319, 92320, 92321, 92322, 92323, 92324, 92331, 92332, 92333, 92334, 92335, 92336, 92337, 92338, - 92339View allHide From: 120Sizes: 5-20 ug labeling, 20-50 ug labelingCatalog #: 92501, 92500, 92502, 92503, 92504, 92505, 92506, 92507, 92508, 92509, 92510, 92511, 92512, 92513, 92514, - 92515View allHide

Biotium s Ready-to-Use DNA Ladders are optimally formulated for loading on GelRed® or GelGreen® pre-cast gels.

1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA. 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA. 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation. 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation. The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation. The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation. The 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1000 bp. The 500 bp band contains more DNA to provide internal orientation. The 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1000 bp. The 500 bp band contains more DNA to provide internal orientation. Biotium implements a Quality System, certified by QAS according to Standard QAS ISO 9001:2015. Certificate No. US3564